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Human VEGF-A ELISA Kits


Microplate kits for sandwich ELISA of human vascular endothelial growth factor (VEGF).

Thermo Scientific Human VEGF ELISA Kits

Assay VEGF-A (human vascular endothelial growth factor) levels in human serum, plasma (heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human VEGF ELISA Kits.

These human VEGF-A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of vascular endothelial growth factor (VEGF-A) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human VEGF-A (vascular endothelial growth factor); see Product Details for additional information
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.3 to 2000pg/mL
  • Sensitivity: < 5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human VEGF ELISA: human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, PLGF-1, PLGF-2, RANTES, TGFβ, TNFβ, VEGF-B, VEGF-C or VEGF-D; rat VEGF; mouse VEGF120 or VEGF164.
Video of the Thermo Scientific VEGF-A ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Human VEGF ELISA standard curve
Human VEGF ELISA standard curve. An example standard curve for the Thermo Scientific Human VEGF ELISA Kit (Part No. EH2VEGF), with error bars based on six replicates for each VEGF standard concentration. Also see standard curves for the VEGF-A Reagent Kit and VEGF-A Antibody Pair Kit.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH2VEGF Human VEGF-A ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human VEGF-A Precoated 96-well Plate, 1 each
Lyophilized Recombinant Human VEGF Standard, 2 vials
Sample Diluent, 14mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 12mL
Streptavidin-HRP Reagent, 75µL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Sealers, 6 each
1-plate kit Product Instructions for product #EH2VEGF Human VEGF-A ELISA Kit MSDS for product #EH2VEGF Human VEGF-A ELISA Kit $399.00

Add to cart
EH2VEGF2 Human VEGF-A ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human VEGF Precoated 96-well Plate, 2 each
Lyophilized Recombinant Human VEGF Standard, 4 vials
Sample Diluent, 2 x 14mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 12mL
Streptavidin-HRP Reagent, 2 x 75µL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Sealers, 12 each
2-plate kit Product Instructions for product #EH2VEGF2 Human VEGF-A ELISA Kit MSDS for product #EH2VEGF2 Human VEGF-A ELISA Kit $650.00

Add to cart
EH2VEGF5 Human VEGF-A ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human VEGF Precoated 96-well Plate, 5 each
Lyophilized Recombinant Human VEGF Standard, 10 vials
Sample Diluent, 5 x 14mL
30X Wash Buffer, 5 x 50mL
Biotinylated Antibody Reagent, 5 x 12mL
Streptavidin-HRP Reagent, 5 x 75µL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Sealers, 30 each
5-plate kit Product Instructions for product #EH2VEGF5 Human VEGF-A ELISA Kit MSDS for product #EH2VEGF5 Human VEGF-A ELISA Kit $1,500.00

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ESSHVEGF Human VEGF-A ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
VEGF-A Coating Antibody, 1 vial
VEGF-A Detection Antibody, 1 vial
Human VEGF Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESSHVEGF Human VEGF-A ELISA Reagent Kit MSDS for product #ESSHVEGF Human VEGF-A ELISA Reagent Kit $375.00

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KHVEGF Human VEGF-A ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
VEGF-A Coating Antibody, 1 vial
VEGF-A Biotinylated Detection Antibody, 1 vial
Human VEGF-A Standard, 1 vial
40-plate kit Product Instructions for product #KHVEGF Human VEGF-A ELISA Antibody Pair Kit MSDS for product #KHVEGF Human VEGF-A ELISA Antibody Pair Kit $999.00

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SVEGF Human VEGF-A Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 02/286
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SVEGF Human VEGF-A Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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