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Human TNF alpha Chemiluminescent ELISA Kits

Sandwich ELISA for human TNF alpha with ECL substrate.

Thermo Scientific Human IL-8 Chemiluminescent ELISA Kits

Assay TNFα (human tumor necrosis factor alpha) levels in serum, plasma (EDTA, heparin) and culture supernatant samples by enhanced chemiluminescence (ECL) with our Thermo Scientific Human TNF alpha Chemiluminescent ELISA Kits.

This human TNF alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate chemiluminescent measurement of of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP and Thermo Scientific SuperSignal Chemiluminescent Substrate solutions. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: natural and recombinant human TNFα (mouse tumor necrosis factor alpha)
  • Format: chemiluminescent ELISA (SuperSignal Substrate) in black 96-well strip plate
  • Measurement: luminometer detection of ECL light emission (max. Em = 425nm)
  • Assay Range: 2 to 20,000pg/mL
  • Sensitivity: < 1.0pg/mL
  • Precision: intra-assay CV < 5%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 2.1 hours
  • Sample Size: 50μL
  • Specificity: cross-reactivity was observed with porcine TNFα; others undetermined
Video of the Thermo Scientific TNF alpha ELISA Kit


Complete kit contains pre-coated antibody-plate, detection antibodies, buffer, diluent, standard, and chemiluminescent substrate.


Human TNF alpha ECL ELISA standard curve
Human TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Human TNF alpha Chemiluminescent ELISA Kit (Part No. 84776), with error bars based on six replicates for each tumor necrosis factor alpha standard concentration.

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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
84776 Human TNF alpha Chemiluminescent ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human TNF alpha Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant TNF alpha Standard, 2 vials
Sample Diluent, 25mL
Biotinylated Antibody Reagent, 7mL
Streptavidin-HRP Reagent, 7mL
SuperSignal Stable Peroxide Solution, 14mL
SuperSignal Luminol/Enhancer Solution, 14mL
Wash Buffer, (30X), 50mL
Plate Sealers, 3 each
1-plate kit Product Instructions for product #84776 Human TNF alpha Chemiluminescent ELISA Kit MSDS for product #84776 Human TNF alpha Chemiluminescent ELISA Kit $550.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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