Update profile
    Change password
    Sign out

Human TNF alpha ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of human TNF alpha.

Thermo Scientific Human TNF alpha ELISA Kits

Assay TNFα (human tumor necrosis factor alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human TNF alpha ELISA Kits.

These human TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human TNFα (human tumor necrosis factor alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 15.6 to 1000pg/mL
  • Sensitivity: < 2pg/mL
  • Precision: dependent upon sample type and level (see instructions)
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNFβ, Eotaxin, RANTES, GROα, GROβ, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF, GMCSF, MIP-1α, MIP-1β, TGFβ, IFNγ, IFNα; mouse TNFα; rat TNFα; and bovine TNFα. TNFα Receptor types 1 and 2 tested at 40mg/mL did not interfere in this assay. Recombinant pig TNFα cross-reacted at 100%.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Video of the Thermo Scientific TNF alpha ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Human TNF alpha ELISA standard curve
Human TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Human TNF alpha ELISA Kit (Part No. EH3TNFA), with error bars based on six replicates for each tumor necrosis factor alpha standard concentration. Also see standard curves for the TNFα Reagent Kit and TNFα Antibody Pair Kit.

TNFα, also called cachectin, macrophage cytotoxin, necrosin, etc., is produced primarily by macrophages in response to bacteria, viruses, mycoplasma, immune complexes, other cytokines, tumor cells, complement and phorbol esters among others. It is a 157 amino acid long polypeptide with an apparent MW of 17 kDa under denaturing conditions but exists as a 45-55 kDa trimer naturally.

In vivo, TNFα has anti-tumor activities against some solid tumors, induces tumorigenesis, metastisis; acts in immunomodulation; induces septic shock, fever; plays a role in multiple sclerosis; and is involved in autoimmunity and infectious disease. TNFα alone and in combination with other cytokines and chemical agents has been used as an anti-tumor agent. Patients with melanoma and other solid tumors, administered high doses of TNFα, have realized a cure rate of up to 90% albeit with some major and minor side effects.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH3TNFA Human TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human TNF alpha Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human TNF alpha Standard, 2 vials
Sample Diluent, 26mL
Biotinylated Antibody Reagent, 13mL
30X Wash Buffer, 50mL
Streptavidin-HRP Reagent, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EH3TNFA Human TNF alpha ELISA Kit MSDS for product #EH3TNFA Human TNF alpha ELISA Kit $495.00

Add to cart
EH3TNFA2 Human TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human TNF alpha Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human TNF alpha Standard, 4 vials
Sample Diluent, 2 x 13mL
Biotinylated Antibody Reagent, 2 x 13mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Reagent, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EH3TNFA2 Human TNF alpha ELISA Kit MSDS for product #EH3TNFA2 Human TNF alpha ELISA Kit $750.00

Add to cart
EH3TNFA5 Human TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human TNF alpha Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human TNF alpha Standard, 5 vials
Sample Diluent, 75mL
Biotinylated Antibody Reagent, 56mL
30X Wash Buffer, 200mL
Streptavidin-HRP Reagent, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EH3TNFA5 Human TNF alpha ELISA Kit MSDS for product #EH3TNFA5 Human TNF alpha ELISA Kit $1,500.00

Add to cart
ESS0001 Human TNF alpha ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
TNF alpha Coating Antibody, 1 vial
TNF alpha Detection Antibody, 1 vial
Human TNF alpha Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0001 Human TNF alpha ELISA Reagent Kit MSDS for product #ESS0001 Human TNF alpha ELISA Reagent Kit $375.00

Add to cart
KHTNFA Human TNF alpha ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
TNF alpha Coating Antibody, 1 vial
TNF alpha Biotinylated Detection Antibody, 1 vial
Human TNF alpha Standard, 1 vial
40-plate kit Product Instructions for product #KHTNFA Human TNF alpha ELISA Antibody Pair Kit MSDS for product #KHTNFA Human TNF alpha ELISA Antibody Pair Kit $999.00

Add to cart
STNFA Human TNF alpha Standard for ELISA
Formulation: Calibrated to NIAID reference lot 87/650
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #STNFA Human TNF alpha Standard for ELISA $110.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747