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Human Soluble ICAM-1 (CD54) ELISA Kits

Microplate kits for sandwich ELISA of human intercellular adhesion molecule 1 (ICAM-1, CD54).

Thermo Scientific Human ICAM-1 CD54 ELISA Kits

Assay ICAM-1 (human soluble ICAM-1, also called CD54) levels in human serum, plasma (EDTA, heparin) and culture supernatant samples with our Thermo Scientific Human Soluble ICAM (CD54) ELISA Kits.

These human sICAM-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54). Also included are calibrated recombinant protein standard, OPD substrate tablets, diluents and wash buffer. The complete kit contains a pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: human soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54)
  • Format: colorimetric ELISA (OPD substrate) in clear 96-well strip plate
  • Measurement: absorbance at 490nm
  • Assay Range: 0.3 to 10ng/mL
  • Sensitivity: < 0.3ng/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 2.5 hours
  • Sample Size: 25μL of 100-fold diluted samples per well
  • Specificity: undetermined


Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.


Human ICAM-1 (CD54) ELISA standard curve
Human ICAM-1 ELISA standard curve. An example standard curve for the Thermo Scientific Human ICAM-1 (CD54) ELISA Kit (Part No. EH5400), with error bars based on six replicates for each ICAM-1 (CD54) standard concentration.


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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
EH5400 Human Soluble ICAM-1 (CD54) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human sICAM-1 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human sICAM-1 Standard, 2 vials
sICAM-1 Conjugate Reagent, 8mL
Sample Diluent, 49mL
OPD Substrate Tablets, 6 each
OPD Substrate Diluent, 30mL
20X Wash Buffer, 55mL
Adhesive Plate Covers, 6 each
1-plate kit MSDS for product #EH5400 Human Soluble ICAM-1 (CD54) ELISA Kit $438.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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