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Pierce Human Rhinovirus 3C Protease (HRV 3C)


Remove fusion protein purification and detection tags.

Pierce HRV 3C Protease

Thermo Scientific Pierce Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.

Highlights:

  • Effective – HRV 3C is designed to remove purification tags from your protein of interest
  • High activity at 4°C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h
  • Flexible – dual-tagged enzyme is compatible with various columns and buffers

Includes:

  • Kit contains protease, cleavage buffer and control protein.

Applications:

  • Removal of purification tags from fusion proteins

Product Details:

Thermo Scientific Pierce HRV 3C Protease activity is comparable to protease from other suppliers
Thermo Scientific Pierce HRV 3C Protease activity is comparable to protease from other suppliers. Pierce HRV 3C Protease and enzymes from other suppliers were incubated with the GST-Syk control protein for 16h at 4°C. The reaction mixtures were then resolved by SDS-PAGE. PreScission™ Protease is from GE Healthcare, Turbo3C™ Protease is from Accelagen and the other HRV 3C Protease is from EMD/Millipore.

Properties of Thermo Scientific Pierce HRV 3C Protease.
Alternative names Human Rhinovirus 3C
Source Escherichia coli (E.coli)
Molecular weight 47.8kD
Form Liquid
Protease type Cysteine
Optimal reaction conditions 50mM Tris-HCl, 150mM NaCl, pH 8.0
Storage -20°C
The enzyme can also be used in a variety of other buffers

 

More Product Data

Related Resources:

Review of GST-tagged proteins
Review of His-tagged proteins

Related Products:

HisPur Cobalt Superflow Agarose
HisPur Ni-NTA Superflow Agarose
Pierce Glutathione Superflow Agarose
Factor Xa Protease
WELQut Protease (on www.thermoscientificbio.com)
T7 Cell-Free Expression Vectors (selected vectors contain HRV 3C cleavage site)
Anti-HRV3C Recognition Site Antibody (on pierce-antibodies.com)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88946 HRV 3C Protease
Formulation: Three-component kit
Sufficient For: Cleavage of 100mg of target protein at 1:200 enzyme-to-substrate ratio

Kit Contents:
HRV3C Protease (2 units/μL), 500uL
Reaction Buffer (10X), 10mL
GST-Syk Fusion Protein (1μg/μL), 25 μL
1K-unit kit Product Instructions for product #88946 HRV 3C Protease MSDS for product #88946 HRV 3C Protease Certificate of Analysis for product #88946 HRV 3C Protease $205.00

Add to cart
88947 HRV 3C Protease
Formulation: Three-component kit
Sufficient For: Cleavage of 1000mg of target protein at 1:200 enzyme-to-substrate ratio

Kit Contents:
HRV3C Protease (2 units/μL), 5mL
Reaction Buffer (10X), 10mL
GST-Syk Fusion Protein (1μg/μL), 25μL
10K-unit kit Product Instructions for product #88947 HRV 3C Protease MSDS for product #88947 HRV 3C Protease Certificate of Analysis for product #88947 HRV 3C Protease $1,845.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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