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Human MIP-1 alpha ELISA Kits


Microplate kits for sandwich ELISA of human MIP-1 alpha (MIP-1a).

Thermo Scientific Human MIP-1 alpha ELISA Kits

Assay MIP-1α (human macrophage inflammatory protein-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human MIP-1 alpha ELISA Kits.

These human MIP-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of macrophage inflammatory protein-1 alpha (MIP-1α) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human MIP-1α (macrophage inflammatory protein-1 alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: < 2.5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human MIP-1β, MIP-1δ, MIP-3α, MIP-3β; mouse MIP-1α, MIP-1β, MIP-1γ, MIP-3β; or rat MIP-3α, MIP-I and MIP-II.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human MIP-1 alpha ELISA standard curve
Human MIP-1α ELISA standard curve. An example standard curve for the Thermo Scientific Human MIP-1α ELISA Kit (Part No. EH2MIP1A), with error bars based on six replicates for each MIP-1 alpha standard concentration.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH2MIP1A Human MIP-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human MIP-1a Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human MIP-1a Standard, 2 vials
Sample Diluent, 25mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 7mL
Streptavidin-HRP Reagent, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EH2MIP1A Human MIP-1 alpha ELISA Kit MSDS for product #EH2MIP1A Human MIP-1 alpha ELISA Kit $480.00

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EH2MIP1A2 Human MIP-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human MIP-1a Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human MIP-1a Standard, 4 vials
Sample Diluent, 2 x 25mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 7mL
Streptavidin-HRP Reagent, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EH2MIP1A2 Human MIP-1 alpha ELISA Kit MSDS for product #EH2MIP1A2 Human MIP-1 alpha ELISA Kit $750.00

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EH2MIP1A5 Human MIP-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human MIP-1a Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human MIP-1a Standard, 10 vials
Sample Diluent, 5 x 25mL
30X Wash Buffer, 5 x 50mL
Biotinylated Antibody Reagent, 5 x 7mL
Streptavidin-HRP Reagent, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EH2MIP1A5 Human MIP-1 alpha ELISA Kit MSDS for product #EH2MIP1A5 Human MIP-1 alpha ELISA Kit $1,775.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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