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Human MCP-1 ELISA Kits


Microplate kits for sandwich ELISA of human MCP-1.

Thermo Scientific Human MCP-1 ELISA Kits

Assay MCP-1 (human monocyte chemoattractant protein-1) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human MCP-1 ELISA Kits.

These human MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human MCP-1 (monocyte chemoattractant protein-1)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: < 10pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human Angiogenin, EGF, FGF-basic, Eotaxin, GM-CSF, GROα, HB-EGF, HGF, IFNα, IFNγ, KGF, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, PLGF-1, RANTES, TNFα, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, and IL-17.
Video of the Thermo Scientific MCP-1 ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human MCP-1 ELISA standard curve
Human MCP-1 ELISA standard curve. An example standard curve for the Thermo Scientific Human MCP-1 ELISA Kit (Part No. EH2MCP1), with error bars based on twelve replicates for each MCP-1 standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH2MCP1 Human MCP-1 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human MCP-1 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human MCP-1 Standard, 2 vials
Standard Diluent, 25mL
Biotinylated Antibody Reagent, 12mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EH2MCP1 Human MCP-1 ELISA Kit MSDS for product #EH2MCP1 Human MCP-1 ELISA Kit $399.00

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EH2MCP12 Human MCP-1 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human MCP-1 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human MCP-1 Standard, 4 vials
Standard Diluent, 2 x 25mL
Biotinylated Antibody Reagent, 2 x 12mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EH2MCP12 Human MCP-1 ELISA Kit MSDS for product #EH2MCP12 Human MCP-1 ELISA Kit $650.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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