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Human IL-8 Chemiluminescent ELISA Kits

Sandwich ELISA for human interleukin-8 (IL-8) with ECL substrate.

Thermo Scientific Human IL-8 Chemiluminescent ELISA Kits

Assay IL-8 (human interleukin-8) levels in human serum, plasma and culture supernatant samples by enhanced chemiluminescence (ECL) with our Thermo Scientific Human IL-8 Chemiluminescent ELISA Kit.

This human IL-8 ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate chemiluminescent measurement of interleukin-8 (IL-8) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP and Thermo Scientific SuperSignal Chemiluminescent Substrate solutions. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: natural and recombinant human interleukin-8 (IL-8)
  • Format: chemiluminescent ELISA (SuperSignal Substrate) in black 96-well strip plate
  • Measurement: luminometer detection of ECL light emission (max. Em = 425nm)
  • Assay Range: 0.5 to 5000pg/mL
  • Sensitivity: < 0.5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 2.1 hours
  • Sample Size: 50μL
  • Specificity: undetermined
Video of the Thermo Scientific IL-8 ELISA Kit


Complete kit contains pre-coated antibody-plate, detection antibodies, buffer, diluent, standard, and chemiluminescent substrate.


Chemiluminescent Human IL-2 ELISA standard curve
Human IL-8 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-8 Chemiluminescent ELISA Kit (Part No. 84774), with error bars based on twelve replicates for each interleukin-8 standard concentration.

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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
84774 Human IL-8 Chemiluminescent ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Standard, 2 vials
Sample Diluent, 25mL
Biotinylated Antibody Reagent, 7mL
Streptavidin-HRP Reagent, 7mL
SuperSignal Stable Peroxide Solution, 14mL
SuperSignal Luminol/Enhancer Solution, 14mL
Wash Buffer, (30X), 50mL
Plate Sealers, 3 each
1-plate kit Product Instructions for product #84774 Human IL-8 Chemiluminescent ELISA Kit MSDS for product #84774 Human IL-8 Chemiluminescent ELISA Kit $550.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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