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Human IL-8 ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of human interleukin-8 (IL-8).

Thermo Scientific Human IL-8 ELISA Kits

Assay IL-8 (human interleukin-8) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-8 ELISA Kits.

These human IL-8 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-8 (IL-8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: < 2pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with with human PF-4, GROα, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFα, IFNα, IFNγ, or GM-CSF.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Video of the Thermo Scientific IL-8 ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

» Compare ELISA Kit formats »

Details:

Human IL-8 ELISA standard curve
Human IL-8 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-8 ELISA Kit (Part No. EH2IL6), with error bars based on five replicates for each interleukin-8 standard concentration. Also see standard curve for the IL-8 Reagent Kit.

Interleukin-8 is a member of the chemokine alpha superfamily of proteins, which contains at least 14 structurally related cytokines. These cytokines exhibit 20-45% homology, are all basic heparin binding proteins and act on various inflammatory cell types. IL-8 binds to specific cell surface receptors causing a rapid rise in cytosolic Ca++ concentration. Apparent biological effects include neutrophil infiltration, T cell infiltration, angiogenesis, synovial inflammation and plasma leakage.

References:

Related Resources:

Related Products:

Looking for the matched antibody pair kit (Part No. KHIL8)?
The original antibody pair kit associated with this ELISA kit has been discontinued. Consider using Cat. No. CHC1303, available at lifetechnologies.com.

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH2IL8 Human IL-8 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL-8 Standard, 2 vials
Standard Diluent, 25mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 8mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EH2IL8 Human IL-8 ELISA Kit MSDS for product #EH2IL8 Human IL-8 ELISA Kit $495.00

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EH2IL82 Human IL-8 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL-8 Standard, 4 vials
Standard Diluent, 2 x 25mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 8mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EH2IL82 Human IL-8 ELISA Kit MSDS for product #EH2IL82 Human IL-8 ELISA Kit $750.00

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EH2IL85 Human IL-8 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL-8 Standard, 5 vials
Standard Diluent, 110mL
30X Wash Buffer, 200mL
Biotinylated Antibody Reagent, 35mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EH2IL85 Human IL-8 ELISA Kit MSDS for product #EH2IL85 Human IL-8 ELISA Kit $1,775.00

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ESS0013 Human IL-8 ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
IL-8 Coating Antibody, 1 vial
IL-8 Detection Antibody, 1 vial
Human IL-8 Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0013 Human IL-8 ELISA Reagent Kit MSDS for product #ESS0013 Human IL-8 ELISA Reagent Kit $375.00

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SIL8 Human IL-8 Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 89/520
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIL8 Human IL-8 Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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