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Human IL-8 ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of human interleukin-8 (IL-8).

Thermo Scientific Human IL-8 ELISA Kits

Assay IL-8 (human interleukin-8) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-8 ELISA Kits.

These human IL-8 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-8 (IL-8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-8 antibody pairs and IL-8 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: < 2pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with with human PF-4, GROα, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFα, IFNα, IFNγ, or GM-CSF.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Video of the Thermo Scientific IL-8 ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Human IL-8 ELISA standard curve
Human IL-8 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-8 ELISA Kit (Part No. EH2IL6), with error bars based on five replicates for each interleukin-8 standard concentration. Also see standard curves for the IL-8 Reagent Kit and IL-8 Antibody Pair Kit.

Interleukin-8 is a member of the chemokine alpha superfamily of proteins, which contains at least 14 structurally related cytokines. These cytokines exhibit 20-45% homology, are all basic heparin binding proteins and act on various inflammatory cell types. IL-8 binds to specific cell surface receptors causing a rapid rise in cytosolic Ca++ concentration. Apparent biological effects include neutrophil infiltration, T cell infiltration, angiogenesis, synovial inflammation and plasma leakage.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH2IL8 Human IL-8 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL-8 Standard, 2 vials
Standard Diluent, 25mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 8mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EH2IL8 Human IL-8 ELISA Kit MSDS for product #EH2IL8 Human IL-8 ELISA Kit $495.00

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EH2IL82 Human IL-8 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL-8 Standard, 4 vials
Standard Diluent, 2 x 25mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 8mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EH2IL82 Human IL-8 ELISA Kit MSDS for product #EH2IL82 Human IL-8 ELISA Kit $750.00

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EH2IL85 Human IL-8 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IL-8 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL-8 Standard, 5 vials
Standard Diluent, 110mL
30X Wash Buffer, 200mL
Biotinylated Antibody Reagent, 35mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EH2IL85 Human IL-8 ELISA Kit MSDS for product #EH2IL85 Human IL-8 ELISA Kit $1,775.00

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ESS0013 Human IL-8 ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
IL-8 Coating Antibody, 1 vial
IL-8 Detection Antibody, 1 vial
Human IL-8 Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0013 Human IL-8 ELISA Reagent Kit MSDS for product #ESS0013 Human IL-8 ELISA Reagent Kit $375.00

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KHIL8 Human IL-8 ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
IL-8 Coating Antibody, 1 vial
IL-8 Biotinylated Detection Antibody, 1 vial
Human IL-8 Standard, 1 vial
40-plate kit Product Instructions for product #KHIL8 Human IL-8 ELISA Antibody Pair Kit MSDS for product #KHIL8 Human IL-8 ELISA Antibody Pair Kit $999.00

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SIL8 Human IL-8 Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 89/520
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIL8 Human IL-8 Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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