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Human IL-4 ELISA Kits


Microplate kits for sandwich ELISA of human IL-4 (IL-4).

Thermo Scientific Human IL-4 ELISA Kits

Assay IL-4 (human interleukin-4) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-4 ELISA Kits.

The human IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1, BSF1) or B-cell growth factor 1 (BCGF-1, BCGF1), in samples at 50µL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 10.24 to 400pg/mL
  • Sensitivity: ≤ 2pg/mL
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50µL
  • Specificity: no significant cross-reaction with human IL-2, IL-6, IL-10, IFNγ, GM-CSF or mouse IL-4.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human IL-4 ELISA standard curve
Human IL-4 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-4 ELISA Kit (Part No. EH3IL4), with error bars based on six replicates for each IL-4 standard concentration.

The Thermo Scientific Human IL-4 ELISA Kit measures human interleukin-4 (hIL-4) in serum, plasma and cell culture supernatants. Produced by activated T-lymphocytes, hIL-4 (15-19kDa) is a glycosylated cytokine involved in the processes of hematopoiesis, allergic inflammation and antibody production. This ELISA kit uses a matched pair of monoclonal antibodies to capture and detect hIL-4 on 96-well plates. The detecting antibody is conjugated to horseradish peroxidase (HRP), which reacts with the TMB substrate to form a colored product that is linear over the assay dynamic range and directly proportional to the amount of hIL-4 present in the sample.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH3IL4 Human IL-4 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-4 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL-4 Standard, 2 vials
Standard Diluent, 26mL
30X Wash Buffer, 50mL
Anti-Human IL-4-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 55mL
Stop Solution, 13mL
Adhesive Plate Covers, 4 each
1-plate kit Product Instructions for product #EH3IL4 Human IL-4 ELISA Kit MSDS for product #EH3IL4 Human IL-4 ELISA Kit $399.00

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EH3IL42 Human IL-4 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IL-4 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL-4 Standards, 4 vials
Standard Diluent, 2 x 26mL
30X Wash Buffer, 2 x 50mL
Anti-Human IL-4-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 55mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 8 each
2-plate kit Product Instructions for product #EH3IL42 Human IL-4 ELISA Kit MSDS for product #EH3IL42 Human IL-4 ELISA Kit $650.00

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EH3IL45 Human IL-4 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IL-4 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL-4 Standards, 10 vials
Standard Diluent, 5 x 26mL
30X Wash Buffer, 5 x 50mL
Anti-Human IL-4-HRP Concentrate, 5 x 75┬ÁL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 55mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 20 each
5-plate kit Product Instructions for product #EH3IL45 Human IL-4 ELISA Kit MSDS for product #EH3IL45 Human IL-4 ELISA Kit $1,500.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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