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Human IL-17A ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of interleukin-17 (IL-17A, CTLA8).

Thermo Scientific Human IL-17A ELISA Kits

Assay IL-17A (human interleukin-17A) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-17A ELISA Kits.

These human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-17A (also called CTLA8)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.25 to 2000pg/mL
  • Sensitivity: ≤ 5pg/mL
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF
Video of the Thermo Scientific IL-17 ELISA Kit

Details:

Human IL-17A ELISA standard curve
Human IL-17A ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-17A ELISA Kit (Part No. EHIL17A), with error bars based on twelve replicates for each IL-17 standard concentration.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIL17A Human IL-17A ELISA Kit
Formulation: Kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human-IL17A Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL17A Standard, 2 vials
Sample Diluent, 14.5mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 8mL
Streptavidin-HRP Concentrate, 75µL
HRP Dilution Buffer, 14mL
TMB Substrate, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHIL17A Human IL-17A ELISA Kit MSDS for product #EHIL17A Human IL-17A ELISA Kit $399.00

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EHIL17A2 Human IL-17A ELISA Kit
Formulation: Kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human-IL17A Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL17A Standard, 4 vials
Sample Diluent, 2 x 14.5mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 8mL
Streptavidin-HRP Concentrate, 2 x 75µL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHIL17A2 Human IL-17A ELISA Kit MSDS for product #EHIL17A2 Human IL-17A ELISA Kit $650.00

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EHIL17A5 Human IL-17A ELISA Kit
Formulation: Kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human-IL17A Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL17A Standard, 10 vials
Sample Diluent, 5 x 14.5mL
30X Wash Buffer, 5 x 50mL
Biotinylated Antibody Reagent, 5 x 8mL
Streptavidin-HRP Concentrate, 5 x 75µL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHIL17A5 Human IL-17A ELISA Kit MSDS for product #EHIL17A5 Human IL-17A ELISA Kit $1,500.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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