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Human IL-17A ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of interleukin-17 (IL-17A, CTLA8).

Thermo Scientific Human IL-17A ELISA Kits

Assay IL-17A (human interleukin-17A) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-17A ELISA Kits.

These human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-17A (also called CTLA8)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.25 to 2000pg/mL
  • Sensitivity: ≤ 5pg/mL
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Video of the Thermo Scientific IL-17 ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

» Compare ELISA Kit formats »

Details:

Human IL-17A ELISA standard curve
Human IL-17A ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-17A ELISA Kit (Part No. EHIL17A), with error bars based on twelve replicates for each IL-17 standard concentration. Also see standard curve for the IL-17A Reagent Kit.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIL17A Human IL-17A ELISA Kit
Formulation: Kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human-IL17A Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL17A Standard, 2 vials
Sample Diluent, 14.5mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 8mL
Streptavidin-HRP Concentrate, 75µL
HRP Dilution Buffer, 14mL
TMB Substrate, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHIL17A Human IL-17A ELISA Kit MSDS for product #EHIL17A Human IL-17A ELISA Kit $399.00

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EHIL17A2 Human IL-17A ELISA Kit
Formulation: Kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human-IL17A Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL17A Standard, 4 vials
Sample Diluent, 2 x 14.5mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 8mL
Streptavidin-HRP Concentrate, 2 x 75µL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHIL17A2 Human IL-17A ELISA Kit MSDS for product #EHIL17A2 Human IL-17A ELISA Kit $650.00

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EHIL17A5 Human IL-17A ELISA Kit
Formulation: Kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human-IL17A Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL17A Standard, 10 vials
Sample Diluent, 5 x 14.5mL
30X Wash Buffer, 5 x 50mL
Biotinylated Antibody Reagent, 5 x 8mL
Streptavidin-HRP Concentrate, 5 x 75µL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHIL17A5 Human IL-17A ELISA Kit MSDS for product #EHIL17A5 Human IL-17A ELISA Kit $1,500.00

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ESS0030 Human IL-17A ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
IL-17 Coating Antibody, 1 vial
IL-17 Detection Antibody, 1 vial
Human IL-17 Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0030 Human IL-17A ELISA Reagent Kit MSDS for product #ESS0030 Human IL-17A ELISA Reagent Kit $375.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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