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Human IL-16 ELISA Kits

Microplate kits for sandwich ELISA of human interleukin-16 (IL-16).

Thermo Scientific Human IL-16 ELISA Kits

Assay IL-16 (human interleukin-16) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-16 ELISA Kits.

These human IL-16 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-16 (IL-16) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: natural and recombinant human interleukin-16 (IL-16)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 0 to 2000pg/mL
  • Sensitivity: < 8pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.25 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human Eotaxin, G-CSF, GM-CSF, GROα, GROβ, IFNα, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-17, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, TNFα, TNFβ, or RANTES.
Video of the Thermo Scientific IL-16 ELISA Kit


Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.


Human IL-16 ELISA standard curve
Human IL-16 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-16 ELISA Kit (Part No. EHIL16), with error bars based on six replicates for each interleukin-16 standard concentration.


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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIL16 Human IL-16 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-16 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL-16 Standard, 2 vials
Standard Diluent, 2 x 25mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHIL16 Human IL-16 ELISA Kit MSDS for product #EHIL16 Human IL-16 ELISA Kit $399.00

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SIL16 Human IL-16 Standard for ELISA
Formulation: Calibrated to Thermo Scientific Internal Reference Standard
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIL16 Human IL-16 Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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