Update profile
    Change password
    Sign out

Human IL-12 (p70) ELISA Kits


Microplate kits for sandwich ELISA of biologically active human interleukin-12 (IL-12 p70).

Thermo Scientific Human IL-12 ELISA Kits

Assay IL-12 p70 (active human interleukin-12, p70) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-12 (p70) ELISA Kits.

These human IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: biologically active (p70) natural and recombinant human interleukin-12 (IL-12, p70)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 15.4 to 600pg/mL
  • Sensitivity: < 3pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, TNFα, IFNγ, GM-CSF, GROα or GROβ. Recombinant human IL-12 (p40) and mouse IL-12 (p40) do not cross-react or interfere with this assay.
Video of the Thermo Scientific IL-12 ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human IL-12 p70 ELISA standard curve
Human active IL-12 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-12 p70 ELISA Kit (Part No. EHIL12), with error bars based on four replicates for each biologically active interleukin-12 p70 standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIL12 Human IL-12 (p70) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-12 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL-12 Standard, 2 vials
Standard Diluent, 14mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 13mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHIL12 Human IL-12 (p70) ELISA Kit MSDS for product #EHIL12 Human IL-12 (p70) ELISA Kit $399.00

Add to cart
EHIL122 Human IL-12 (p70) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IL-12 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL-12 Standard, 4 vials
Standard Diluent, 2 x 14mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 13mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHIL122 Human IL-12 (p70) ELISA Kit MSDS for product #EHIL122 Human IL-12 (p70) ELISA Kit $650.00

Add to cart
EHIL125 Human IL-12 (p70) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IL-12 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL-12 Standards, 5 vials
Standard Diluent, 75mL
Biotinylated Antibody Reagent, 35mL
30X Wash Buffer, 200mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHIL125 Human IL-12 (p70) ELISA Kit MSDS for product #EHIL125 Human IL-12 (p70) ELISA Kit $1,500.00

Add to cart
SIL12 Human IL-12 (p70) Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 95/544
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIL12 Human IL-12 (p70) Standard for ELISA $110.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747