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Human IL-10 ELISA Kits


Microplate kits for sandwich ELISA of human interleukin-10 (IL-10).

Thermo Scientific Human IL-10 ELISA Kits

Assay IL-10 (human interleukin-10) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-10 ELISA Kits.

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-10 (IL-10)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 15.4 to 600pg/mL
  • Sensitivity: < 3pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ, or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10.
Video of the Thermo Scientific IL-10 ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human IL-10 ELISA standard curve
Human IL-10 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-10 ELISA Kit (Part No. EHIL10), with error bars based on six replicates for each interleukin-10 standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIL10 Human IL-10 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-10 Precoated 96-well Strip Plate, 1 each
Recombinant Human IL-10 Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
1-plate kit Product Instructions for product #EHIL10 Human IL-10 ELISA Kit MSDS for product #EHIL10 Human IL-10 ELISA Kit $399.00

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EHIL102 Human IL-10 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IL-10 Precoated 96-well Strip Plate, 2 each
Recombinant Human IL-10 Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHIL102 Human IL-10 ELISA Kit MSDS for product #EHIL102 Human IL-10 ELISA Kit $650.00

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EHIL105 Human IL-10 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IL-10 Precoated 96-well Strip Plate, 5 each
Recombinant Human IL-10 Standard, 10 vials
Standard Diluent, 5 x 12mL
Biotinylated Antibody Reagent, 5 x 8mL
30X Wash Buffer, 5 x 50mL
Streptavidin-HRP Concentrate, 5 x 75┬ÁL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHIL105 Human IL-10 ELISA Kit MSDS for product #EHIL105 Human IL-10 ELISA Kit $1,500.00

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SIL10 Human IL-10 Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 93/722
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIL10 Human IL-10 Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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