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Human IL-1 alpha ELISA Kits


Microplate sandwich ELISA kits for human interleukin 1 alpha (IL-1a).

Thermo Scientific Human IL-1 alpha ELISA Kits

Assay IL-1α (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-1 alpha ELISA Kits.

These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interleukin-1 alpha (IL-1α)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 10.2 to 400pg/mL
  • Sensitivity: < 2pg/mL
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA.
Video of the Thermo Scientific IL-1 alpha ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human IL-1 alpha ELISA standard curve
Human IL-1α ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-1α ELISA Kit (Part No. EH2IL1A), with error bars based on five replicates for each interleukin-1 alpha standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EH2IL1A Human IL-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IL-1 alpha Standard, 2 vials
Standard Diluent, 12mL, contains 0.01% thimerosal
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EH2IL1A Human IL-1 alpha ELISA Kit MSDS for product #EH2IL1A Human IL-1 alpha ELISA Kit $399.00

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EH2IL1A2 Human IL-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IL-1 alpha Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EH2IL1A2 Human IL-1 alpha ELISA Kit MSDS for product #EH2IL1A2 Human IL-1 alpha ELISA Kit $650.00

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EH2IL1A5 Human IL-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL-1 alpha Standard, 10 vials
Standard Diluent, 5 x 12mL
Biotinylated Antibody Reagent, 5 x 8mL
30X Wash Buffer, 5 x 50mL
Streptavidin-HRP Concentrate, 5 x 75┬ÁL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EH2IL1A5 Human IL-1 alpha ELISA Kit MSDS for product #EH2IL1A5 Human IL-1 alpha ELISA Kit $1,500.00

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SIL1A Human IL-1 alpha Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 86/632
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIL1A Human IL-1 alpha Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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