Update profile
    Change password
    Sign out

Human IFN gamma ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of human interferon gamma (IFN-gamma).

Thermo Scientific Human IFN gamma ELISA Kits

Assay IFN-γ (human interferon gamma) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human IFN gamma ELISA Kits.

These human IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human interferon gamma (IFNγ)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: ≤ 2pg/mL
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, TNFα or GM-CSF

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Video of the Thermo Scientific IFN gamma ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Human IFN gamma ELISA standard curve
Human IFNγ ELISA standard curve. An example standard curve for the Thermo Scientific Human IFNγ ELISA Kit (Part No. EHIFNG), with error bars based on six replicates for each interferon gamma standard concentration. Also see standard curves for the IFNγ Reagent Kit and IFNγ Antibody Pair Kit.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIFNG Human IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human IFN gamma Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human IFN gamma Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHIFNG Human IFN gamma ELISA Kit MSDS for product #EHIFNG Human IFN gamma ELISA Kit $399.00

Add to cart
EHIFNG2 Human IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human IFN gamma Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human IFN gamma Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHIFNG2 Human IFN gamma ELISA Kit MSDS for product #EHIFNG2 Human IFN gamma ELISA Kit $650.00

Add to cart
EHIFNG5 Human IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human IFN gamma Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IFN gamma Standard, 5 vials
Standard Diluent, 75mL
Biotinylated Antibody Reagent, 35mL
30X Wash Buffer, 200mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHIFNG5 Human IFN gamma ELISA Kit MSDS for product #EHIFNG5 Human IFN gamma ELISA Kit $1,775.00

Add to cart
ESS0002 Human IFN gamma ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
IFN gamma Coating Antibody, 1 vial
IFN gamma Detection Antibody, 1 vial
Human IFN gamma Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0002 Human IFN gamma ELISA Reagent Kit MSDS for product #ESS0002 Human IFN gamma ELISA Reagent Kit $375.00

Add to cart
KHIFNG Human IFN gamma ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
IFN gamma Coating Antibody, 1 vial
IFN gamma Biotinylated Detection Antibody, 1 vial
Human IFN gamma Standard, 1 vial
40-plate kit MSDS for product #KHIFNG Human IFN gamma ELISA Antibody Pair Kit $999.00

Add to cart
SIFNG Human IFN gamma Standard for ELISA
Formulation: Recombinant human IFN gamma from E. coli; Lyophilized powder; contains BSA; Calibrated to NIAID reference lot Gxg-01-902-535
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SIFNG Human IFN gamma Standard for ELISA $110.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
89876 - Pierce Antibody-Based Albumin/IgG Removal Kit

78430 - Halt Protease Inhibitor Single-Use Cocktail (100X)

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747