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Human IFN alpha ELISA Kit (Serum)


Microplate sandwich ELISA kits for human interferon alpha (IFN-alpha).

VeriKine Human IFN alpha ELISA Kits

Assay IFN-α (human interferon alpha) levels in human serum, plasma and tissue culture media with these VeriKine* Human IFN alpha ELISA Kits (for Serum Samples).

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 50μL per well. These IFN-α kits are validated for use with human serum, plasma and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: human interferon alpha (human IFN-α, IFN-alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
  • Measurement: absorbance at 450nm
  • Assay Range: 12.5 to 1000pg/mL
  • Sensitivity: undetermined
  • Precision: intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
  • Sample Types: cell culture supernatant, serum, plasma
  • Total Assay Time: 3.25 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β, or IFN-γ; bovine IFN-τ.
Video of the Thermo Scientific IFN alpha ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.

Details:

Human IFN alpha ELISA standard curve
Human IFN-α ELISA standard curve. An example standard curve for the VeriKine* Human IFN-α ELISA Kit (Part No. 411101), with error bars based on three replicates for each interferon alpha standard concentration. This graph represents the standard prepared in Standard Diluent; also see the IFNα standard curve prepared in serum.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
411101 Human IFN alpha ELISA Kit (Serum Samples)
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Precoated 96-well Plate, 1 each
Plate Sealers, 4 ea.
Wash Solution Concentrate, 50mL
Human IFN Alpha Standard 10,000pg/mL, 1 vial
Sample Diluent, 30mL
Antibody Concentrate, 1 vial
HRP Conjugate Concentrate, 1 vial
Concentrate Diluent, 15mL
TMB Substrate Solution, 15mL
Stop Solution, 15mL
Dilution Buffer, 30mL
1-plate kit MSDS for product #411101 Human IFN alpha ELISA Kit (Serum Samples) $599.00

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411102 Human IFN alpha ELISA Kit (Serum Samples)
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Precoated 96-well Plates, 5 each
Plate Sealers, 20 ea.
Wash Solution Concentrate, 250mL
Human IFN Alpha Standard 10,000pg/mL, 2.5mL
Sample Diluent, 150mL
Antibody Concentrate, 1 vial
HRP Conjugate Concentrate, 1 vial
Concentrate Diluent, 60mL
TMB Substrate Solution, 60mL
Stop Solution, 60mL
Dilution Buffer, 150mL
5-plate kit MSDS for product #411102 Human IFN alpha ELISA Kit (Serum Samples) $2,150.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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