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Human I-TAC ELISA Kits


Microplate sandwich ELISA kits for human I-TAC (CXCL11).

Thermo Scientific Human I-TAC ELISA Kits

Assay I-TAC (human interferon-inducible T-cell alpha chemoattractant) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human I-TAC ELISA Kits.

These human I-TAC ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of I-TAC (also known as CXCL11 or IP9) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human I-TAC (human interferon-inducible T-cell alpha chemoattractant, also called CXCL11, IP9)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 41.0 to 4000pg/mL
  • Sensitivity: < 5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human MIG/CXCL9, IP-10/CXCL10, IL-8, IFNα, or mouse ITAC/CXCL11.
Video of the Thermo Scientific I-TAC ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human I-TAC ELISA standard curve
Human I-TAC ELISA standard curve. An example standard curve for the Thermo Scientific Human I-TAC ELISA Kit (Part No. EHITAC), with error bars based on 12 replicates for each ITAC standard concentration.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHITAC Human ITAC ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human I-TAC Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human I-TAC Standard, 2 vials
Standard Diluent, 28mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 8mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHITAC Human ITAC ELISA Kit MSDS for product #EHITAC Human ITAC ELISA Kit $399.00

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EHITAC2 Human ITAC ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human I-TAC Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human I-TAC Standards, 4 vials
Standard Diluent, 2 x 28mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 8mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHITAC2 Human ITAC ELISA Kit MSDS for product #EHITAC2 Human ITAC ELISA Kit $650.00

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EHITAC5 Human ITAC ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human I-TAC Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human I-TAC Standard, 5 vials
Standard Diluent, 110mL
30X Wash Buffer, 200mL
Biotinylated Antibody Reagent, 35mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHITAC5 Human ITAC ELISA Kit MSDS for product #EHITAC5 Human ITAC ELISA Kit $1,400.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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