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Human HIF-1 alpha ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of hypoxia-inducible factor 1-alpha (HIF1A).

Thermo Scientific HIF-1 alpha ELISA Kits

Assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and cell culture lysate samples with our Thermo Scientific HIF-1 alpha ELISA Kits.

These HIF-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of hypoxia-inducible factor 1-alpha (HIF1A, also called BHLHE78, MOP1, PASD8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:†

  • Target: natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 81.92 to 20,000pg/mL
  • Sensitivity: < 30pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture lysate, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A.

† Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

» Compare ELISA Kit formats »

Details:

Human HIF-1 alpha ELISA standard curve
Human HIF-1 alpha ELISA standard curve. An example standard curve for the Thermo Scientific HIF-1 Alpha ELISA Kit (Part No. EHIF1A), with error bars based on two replicates for each epidermal growth factor standard concentration.

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Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHIF1A Human HIF-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-HIF-1A Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant HIF-1A Standard, 2 vials
Standard Diluent, 14mL
30X Wash Buffer, 50mL
Biotinylated Antibody Reagent, 12mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHIF1A Human HIF-1 alpha ELISA Kit MSDS for product #EHIF1A Human HIF-1 alpha ELISA Kit $399.00

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EHIF1A2 Human HIF-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-HIF-1A Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant HIF-1A Standard, 4 vials
Standard Diluent, 2 x 14mL
30X Wash Buffer, 2 x 50mL
Biotinylated Antibody Reagent, 2 x 12mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHIF1A2 Human HIF-1 alpha ELISA Kit MSDS for product #EHIF1A2 Human HIF-1 alpha ELISA Kit $650.00

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EHIF1A5 Human HIF-1 alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-HIF-1A Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant HIF-1A Standard, 10 vials
Standard Diluent, 5 x 14mL
30X Wash Buffer, 5 x 50mL
Biotinylated Antibody Reagent, 5 x 12mL
Streptavidin-HRP Concentrate, 5 x 75┬ÁL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHIF1A5 Human HIF-1 alpha ELISA Kit MSDS for product #EHIF1A5 Human HIF-1 alpha ELISA Kit $1,500.00

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ESSHIF1A Human HIF-1 alpha ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
HIF-1A Coating Antibody, 1 vial
HIF-1A Detection Antibody, 1 vial
Lyophilized Recombinant HIF-1A Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESSHIF1A Human HIF-1 alpha ELISA Reagent Kit MSDS for product #ESSHIF1A Human HIF-1 alpha ELISA Reagent Kit $375.00

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SHIF1A Human HIF-1 alpha Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 91/550
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SHIF1A Human HIF-1 alpha Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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