Update profile
    Change password
    Sign out

Human GM-CSF ELISA Kit


Microplate sandwich ELISA kits for human GM-CSF.

Thermo Scientific Human GM-CSF beta ELISA Kits

Assay GM-CSF (granulocyte macrophage colony-stimulating factor) levels in human serum, plasma, urine and culture supernatants with our Thermo Scientific Human GM-CSF ELISA Kits.

These human GM-CSF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of granulocyte macrophage colony-stimulating factor (GM-CSF) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 15.4 to 600pg/mL
  • Sensitivity: < 2pg/mL
  • Reproducibility: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, IFNγ, TNFα, or mouse GM-CSF.
Video of the Thermo Scientific Human GM-CSF ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human GM-CSF ELISA standard curve
Human GM-CSF ELISA standard curve. An example standard curve for the Thermo Scientific Human GM-CSF ELISA Kit (Part No. EHGMCSF), with error bars based on six replicates for each granulocyte macrophage colony-stimulating factor standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHGMCSF Human GM-CSF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human GM-CSF Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human GM-CSF Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHGMCSF Human GM-CSF ELISA Kit MSDS for product #EHGMCSF Human GM-CSF ELISA Kit $399.00

Add to cart
EHGMCSF2 Human GM-CSF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human GM-CSF Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human GM-CSF Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75µL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHGMCSF2 Human GM-CSF ELISA Kit MSDS for product #EHGMCSF2 Human GM-CSF ELISA Kit $650.00

Add to cart
EHGMCSF2 Human GM-CSF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human GM-CSF Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human GM-CSF Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75µL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHGMCSF2 Human GM-CSF ELISA Kit MSDS for product #EHGMCSF2 Human GM-CSF ELISA Kit $650.00

Add to cart
EHGMCSF5 Human GM-CSF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human GM-CSF Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human GM-CSF Standard, 10 vials
Standard Diluent, 5 x 12mL
Biotinylated Antibody Reagent, 5 x 8mL
30X Wash Buffer, 5 x 50mL
Streptavidin-HRP Concentrate, 5 x 75µL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHGMCSF5 Human GM-CSF ELISA Kit MSDS for product #EHGMCSF5 Human GM-CSF ELISA Kit $1,500.00

Add to cart
SGMCSF Human GM-CSF Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 88/646
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SGMCSF Human GM-CSF Standard for ELISA $110.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
37528 - Blocker Casein in PBS

37532 - Blocker Casein in TBS

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747