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Human EGF ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of human epidermal growth factor (EGF).

Thermo Scientific Human EGF ELISA Kits

Assay EGF (epidermal growth factor) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human EGF ELISA Kits.

These human EGF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of epidermal growth factor (EGF, also called URG, HOMG4) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched EGF antibody pairs and EGF standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 7.8 to 500pg/mL
  • Sensitivity: ≤ 5pg/mL
  • Reproducibility: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFα TGFβ, GM-CSF

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Video of the Thermo Scientific EGF ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Human EGF ELISA standard curve
Human EGF ELISA standard curve. An example standard curve for the Thermo Scientific Human EGF ELISA Kit (Part No. EHIFNG), with error bars based on two replicates for each epidermal growth factor standard concentration. Also see standard curves for the EGF Reagent Kit and EGF Antibody Pair Kit.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EHEGF Human EGF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Human EGF Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Human EGF Standard, 2 vials
Standard Diluent, 14mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EHEGF Human EGF ELISA Kit MSDS for product #EHEGF Human EGF ELISA Kit $399.00

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EHEGF2 Human EGF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Human EGF Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Human EGF Standards, 4 vials
Standard Diluent, 2 x 14mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EHEGF2 Human EGF ELISA Kit MSDS for product #EHEGF2 Human EGF ELISA Kit $650.00

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EHEGF5 Human EGF ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Human EGF Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human EGF Standard, 5 vials
Standard Diluent, 75mL
Biotinylated Antibody Reagent, 35mL
30X Wash Buffer, 200mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EHEGF5 Human EGF ELISA Kit MSDS for product #EHEGF5 Human EGF ELISA Kit $1,500.00

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ESSHEGF Human EGF ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
EGF Coating Antibody, 1 vial
EGF Detection Antibody, 1 vial
Human EGF gamma Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESSHEGF Human EGF ELISA Reagent Kit MSDS for product #ESSHEGF Human EGF ELISA Reagent Kit $375.00

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KHEGF Human EGF ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
EGF Coating Antibody, 1 vial
EGF Biotinylated Detection Antibody, 1 vial
HUman EGF Standard, 1 vial
40-plate kit Product Instructions for product #KHEGF Human EGF ELISA Antibody Pair Kit MSDS for product #KHEGF Human EGF ELISA Antibody Pair Kit $999.00

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SEGF Human EGF Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 91/550
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SEGF Human EGF Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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