HRP-Conjugated Streptavidin consists of streptavidin protein that is covalently conjugated to horseradish peroxidase (HRP) enzyme (RZ > 3.0). Streptavidin binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate substrate system. This particular product has been used primarily in sandwich ELISA applications to provide consistent measurement of biotinylated detection antibodies.
- Product Number: N100
- Size: 0.25mL
- Concentration: 1.25 mg/mL
- Formulation: Provided in a conjugate stabilizer containing 0.1% ProClin Compound as a preservative.
- Storage: Shipped on cold packs. Store at 2-8°C upon receipt. Protect from exposure to light. Stable until expiration date if stored under these conditions.
- Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is from 1:4,000 to 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.
Blocking Buffer, Stop Solution, and TMB Substrate
Poly-HRP Streptavidin for ELISA (Product # N200)
High-Senstivity Streptavidin-HRP Conjugates