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Activated Peroxidase and Antibody Labeling Kit


Glutaraldehyde-activated for amine conjugation.

Pierce Activated Peroxidase and Antibody Labeling Kit

The Thermo Scientific Pierce Activated Peroxidase and Antibody Labeling Kit are designed to prepare and purify HRP-conjugated antibodies with any previously unmodified primary or secondary antibody (IgG).

Activated Peroxidase is HRP enzyme that has been activated with glutaraldehyde to contain reactive groups that will spontaneously conjugate with amines on an antibody (or other protein). Incubation of this Activated Peroxidase with IgG in sodium carbonate buffer (pH 9.5) results in formation of a conjugate where both the enzyme activity of HRP and the antigen-binding activity of IgG are preserved. The Antibody Labeling Kit includes the Activated Peroxidase and a Protein A/G column and buffers to purify the antibody conjugate from excess, unreacted HRP enzyme.

Highlights:

  • Activated HRP – glutaraldehyde-activated horseradish peroxidase, ready for conjugation to antibodies and other proteins at sites of primary amines (e.g., lysines)
  • Permanent conjugation – reacts with primary amines to form covalent amide bonds; no reduction step is necessary to secure the linkage
  • High activity HRP – enzyme activity is greater than 200 units/mg; lyophilized, activated enzyme is stable for at least 12 months at -20°C
  • Convenient quantities – each 1mg-quantity of activated enzyme in the kit is sufficient for reaction with 0.3mg of IgG to produce about 0.5mL of conjugate

Product Details:

For additional information about glutaraldehyde conjugation, see our review of Reductive Amination and the discussion of applications that follows.

References:

  1. Imagawa, M., et al. (1982) Characteristics and evaluation of antibody horseradish peroxidase conjugates prepared by using a maleimide compound, gluteraldehyde and periodate. J. Appl. Biochem. 4, 41-57.

Related Resources:

Immunohistochemistry (IHC) technical guide
Immunodetection for IHC technical guide

Related Products:

Plus Activated Peroxidase Kit – more effective coupling but does not include antibody purification tools
Anti-HRP Monoclonal Antibody (Part No. MA1-10371)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
31497 EZ-Link Activated Peroxidase Antibody Labeling Kit
Formulation: Multi-component kit
Sufficient For: 5 conjugations, each with 0.3mg antibody (IgG)

Kit Contents:
Activated Peroxidase, 5mg
Conjugation Buffer, 50mL
Lysine, 250mg
Protein A/G Column, 0.5mL
Gentle Ag/Ab Binding Buffer, 200mL
Gentle Ag/Ab Elution Buffer, 200mL
5-rxn kit Product Instructions for product #31497 EZ-Link Activated Peroxidase Antibody Labeling Kit MSDS for product #31497 EZ-Link Activated Peroxidase Antibody Labeling Kit Certificate of Analysis for product #31497 EZ-Link Activated Peroxidase Antibody Labeling Kit $534.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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