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HPG (p-Hydroxyphenylglyoxal)


Effective reagent for modifying and detecting arginine residues.

Pierce HPG (p-hydroxyphenylglyoxal)

Thermo Scientific Pierce HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.

Highlights:

  • Arginine-specific – reacts specifically with arginine residues under mild conditions (pH 7 to 9, 25°C)
  • Quantitative – reaction follows Beer's Law at 5 to 50µM and can be monitored at 340nm (pH 9)
  • Superior to alternatives – more resistant to oxidation than p-nitrophenylglyoxal and more water-soluble than phenylglyoxal

Reagent Properties:

  • Alternative names: HPG monohydrate
  • Chemical name: (p-Hydroxyphenyl)glyoxal; (4-Hydroxyphenyl)(oxo)acetaldehyde
  • Reactive toward: Arginine (Arg, R)
  • Chemical formula: C8H6O3
  • CAS number: 197447-05-5; 24645-80-5
  • Molecular weight: 150.13 (supplied as monohydrate: 168.15)
  • Form: Off-white crystalline powder

Product Details:

Chemical structure of HPG p-hydroxyphenylglyoxal Chemical structure of p-hydroxphenylglyoxal (HPG).

References:

  1. Yamasaki, R.B., et al. (1980). Anal. Biochem. 109, 32-40.

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20100 HPG (p-Hydroxyphenylglyoxal)
Formulation: p-Hydroxyphenylglyoxal; crystalline powder (at least 85% pure)
Sufficient For: Modifying approx. 1mg peptide per 1mg reagent (100mg total)
100mg MSDS for product #20100 HPG (p-Hydroxyphenylglyoxal) Certificate of Analysis for product #20100 HPG (p-Hydroxyphenylglyoxal) $153.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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