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Heptafluorobutyric Acid (HFBA)


Ion-pair reagent for the reverse-phase HPLC separation of proteins and peptides.

Pierce Heptafluorobutyric Acid (HFBA)

Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Pierce HFBA is highly purified and stably packaged heptafluorobutyric acid that is tested and prepared for use as an ion-pairing reagent in high-pressure liquid chromatography (HPLC) methods and similar applications. The solution is tested for overall purity, chain-length purity, water content, sulfate content and absorptivity at three different UV-wavelengths. The liquid reagent is offered in convenient 1mL ampules and 100mL bottles.

Highlights:

  • Greater than 99.5% purity – allows sensitive, nondestructive peptide detection at low UV wavelengths in reverse-phase HPLC protein and peptide separation systems
  • High-performance packaging – HFBA packaged under nitrogen in amber glass ampules or bottles
  • Economical convenience – in just a few seconds, a 1mL ampule can be used to prepare 1 liter of fresh 0.1% heptafluorobutyric acid solution for the mobile phase in reverse-phase chromatography

Applications:

  • Ion pair reagent for reverse-phase HPLC
  • Protein/peptide sequencing
  • Protein/peptide solubilizing agent
  • Solid-phase peptide synthesis
  • Amino acid analysis

Product Details:

Chemical structure of HFBA, heptafluorobutyric acid

Properties of heptafluorobutyric acid.
Molecular formula C4HF7O2
Molecular weight 214.04
Density 1.64g/mL
CAS number 375-22-4

Pierce HFBA is manufactured to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Specifications of Thermo Scientific Pierce HFBA.
Purity > 99.5%
Water content < 0.1%
Chain length > 99.5% C4
UV (neat) A280 < 0.004
A254 < 0.006
A230 < 0.150
Sulfate Negative

References:

  1. Hermann, P.M., et al. (2000) J. Neurosci. 20, 6355-6364.
  2. Hearn, M.T.W. and Hancock, W.S. (1979). Trends in Biochemical Sciences 4, N58-N62.
  3. Bennett, H.P.J., et al. (1980). J. Liquid Chromatogr. 3, 1353-1365.
  4. Bennett, H.P.J., et al. (1981). Biochem. 20, 4530-4538.

Related Products:

Dimethylsulfoxide (DMSO)
Trifluoroacetic acid (TFA)
Triethylamine
Acetonitrile
Water
Amino Acid Detection Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
25003 Heptafluorobutyric Acid (HFBA), Sequencing Grade
Formulation: Clear, colorless liquid of HFBA (at least 99.5% pure; zero sulfate; low water; low absorbance)
Sufficient For: Sequencing and liquid chromatography applications
100mL MSDS for product #25003 Heptafluorobutyric Acid (HFBA), Sequencing Grade Certificate of Analysis for product #25003 Heptafluorobutyric Acid (HFBA), Sequencing Grade $352.00

Add to cart
53104 Heptafluorobutyric Acid (HFBA), HPLC Grade
Formulation: Clear, colorless liquid of HFBA (at least 99.5% pure; zero sulfate; low water; low absorbance)
Sufficient For: Sequencing and liquid chromatography applications
10 x 1mL MSDS for product #53104 Heptafluorobutyric Acid (HFBA), HPLC Grade Certificate of Analysis for product #53104 Heptafluorobutyric Acid (HFBA), HPLC Grade $105.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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