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Heavy and Light Amino Acids for SILAC Labeling


Arginine, lysine, leucine and proline for stable isotope labeling in cell culture.

Heavy and Light Amino Acids for SILAC labeling

Thermo Scientific Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of "heavy" 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells
  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases
  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment
  • High-quality supplements – heavy amino acids with >99% isotope purity

Product Details:

Thermo Scientific Heavy and Light Amino Acids for SILAC are used together with specialized cell culture media that are deficient in essential amino acids. Heavy and light L-lysine and L-arginine are the most common amino acids used for SILAC analysis of tryptic peptides. Up to three different experimental conditions can be readily analyzed with different isotopes of lysine and arginine. For lysine three-plex experiments, 4,4,5,5-D4 L-lysine and 13C6 15N2 L-lysine are used to generate peptides with 4- and 8-Da mass shifts, respectively, compared to peptides generated with light lysine. For arginine three-plex experiments, 13C6 L-arginine and 13C6 15N4 L-arginine are used to generate peptides with 6- and 10-Da mass shifts, respectively, compared to peptides generated with light arginine. L-leucine is another amino acid commonly used for SILAC labeling, because it is one of the most common amino acids found in protein sequences. Proline is a non-essential amino acid that is sometimes added to SILAC media to prevent the metabolic conversion of heavy arginine to heavy proline in mammalian cell lines with high arginine dehydrogenase activity.

Related Resources:

SILAC Protein Quantitation Kit FAQ
Tech Tip #60: Prepare SILAC peptides using the In-Gel Tryptic Digestion Kit
Review of Quantitative Proteomics
Service Providers that use our SILAC Reagents

Related Products:

SILAC Protein Quantitation Kits – complete SILAC kits for DMEM or RPMI 1640 media
SILAC Media and Dialyzed FBS – amino acid-deficient SILAC media and components
Heavy Protein In Vitro Expression Kits – expess proteins that incorporate heavy amino acids
In-Gel Trypsin Digest Kit – optimized kit for protein MS sample preparation


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89989 L-Arginine-HCl
Formulation: Lyophilized powder of L-Arginine-HCl (at least 98% pure)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #89989 L-Arginine-HCl Certificate of Analysis for product #89989 L-Arginine-HCl $29.00

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88427 L-Arginine-HCl
Formulation: Lyophilized powder of L-Arginine-HCl (at least 98% pure)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88427 L-Arginine-HCl Certificate of Analysis for product #88427 L-Arginine-HCl $103.00

Add to cart
88210 L-Arginine-HCl, 13C6
Formulation: Lyophilized powder of L-Arginine-HCl (at least 99% 13C6)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #88210 L-Arginine-HCl, 13C6 Certificate of Analysis for product #88210 L-Arginine-HCl, 13C6 $372.00

Add to cart
88433 L-Arginine-HCl, 13C6
Formulation: Lyophilized powder of L-Arginine-HCl (at least 99% 13C6)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88433 L-Arginine-HCl, 13C6 Certificate of Analysis for product #88433 L-Arginine-HCl, 13C6 $2,255.00

Add to cart
89990 L-Arginine-HCl, 13C6, 15N4
Formulation: Lyophilized powder of L-Arginine-HCl (at least 98% 13C6, 15N4)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #89990 L-Arginine-HCl, 13C6, 15N4 Certificate of Analysis for product #89990 L-Arginine-HCl, 13C6, 15N4 $345.00

Add to cart
88434 L-Arginine-HCl, 13C6, 15N4
Formulation: Lyophilized powder of L-Arginine-HCl (at least 98% 13C6, 15N4)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88434 L-Arginine-HCl, 13C6, 15N4 Certificate of Analysis for product #88434 L-Arginine-HCl, 13C6, 15N4 $2,255.00

Add to cart
89987 L-Lysine-2HCl
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 98.5% pure)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #89987 L-Lysine-2HCl Certificate of Analysis for product #89987 L-Lysine-2HCl $29.00

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88429 L-Lysine-2HCl
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 98.5% pure)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88429 L-Lysine-2HCl Certificate of Analysis for product #88429 L-Lysine-2HCl $103.00

Add to cart
89988 L-Lysine-2HCl, 13C6
Formulation: Lyophilized powder of L-Lysine-HCl (at least 98% 13C6)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #89988 L-Lysine-2HCl, 13C6 Certificate of Analysis for product #89988 L-Lysine-2HCl, 13C6 $345.00

Add to cart
88431 L-Lysine-2HCl, 13C6
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 99% 13C6)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88431 L-Lysine-2HCl, 13C6 Certificate of Analysis for product #88431 L-Lysine-2HCl, 13C6 $2,255.00

Add to cart
88209 L-Lysine-2HCl, 13C6, 15N2
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 99% 13C6, 15N2)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #88209 L-Lysine-2HCl, 13C6, 15N2 Certificate of Analysis for product #88209 L-Lysine-2HCl, 13C6, 15N2 $345.00

Add to cart
88432 L-Lysine-2HCl, 13C6, 15N2
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 99% 13C6, 15N2)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88432 L-Lysine-2HCl, 13C6, 15N2 Certificate of Analysis for product #88432 L-Lysine-2HCl, 13C6, 15N2 $2,255.00

Add to cart
88437 L-Lysine-2HCl, 4,4,5,5-D4
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 96% 4,4,5,5-D4)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #88437 L-Lysine-2HCl, 4,4,5,5-D4 Certificate of Analysis for product #88437 L-Lysine-2HCl, 4,4,5,5-D4 $103.00

Add to cart
88438 L-Lysine-2HCl, 4,4,5,5-D4
Formulation: Lyophilized powder of L-Lysine-2HCl (at least 96% 4,4,5,5-D4)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88438 L-Lysine-2HCl, 4,4,5,5-D4 Certificate of Analysis for product #88438 L-Lysine-2HCl, 4,4,5,5-D4 $646.00

Add to cart
88428 L-Leucine
Formulation: Lyophilized powder of L-Leucine (at least 98.5% pure)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88428 L-Leucine Certificate of Analysis for product #88428 L-Leucine $103.00

Add to cart
88435 L-Leucine, 13C6
Formulation: Lyophilized powder of L-Leucine (at least 99% 13C6)
Sufficient For: Media for 1 SILAC experiment
50mg MSDS for product #88435 L-Leucine, 13C6 Certificate of Analysis for product #88435 L-Leucine, 13C6 $379.00

Add to cart
88436 L-Leucine, 13C6
Formulation: Lyophilized powder of L-Leucine (at least 99% 13C6)
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88436 L-Leucine, 13C6 Certificate of Analysis for product #88436 L-Leucine, 13C6 $2,255.00

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88211 L-Proline
Formulation: Crystalline powder of L-Proline
Sufficient For: Media for 1 SILAC experiment
115mg MSDS for product #88211 L-Proline Certificate of Analysis for product #88211 L-Proline $29.00

Add to cart
88430 L-Proline
Formulation: Crystalline powder of L-Proline
Sufficient For: Media for 10 SILAC experiments
500mg MSDS for product #88430 L-Proline Certificate of Analysis for product #88430 L-Proline $103.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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