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Halt Protease and Phosphatase Inhibitor Cocktail


Complete protection from proteases and phosphatases in one reliable cocktail.

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail

The all-in-one Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktails provide the convenience of a single solution with full protein sample protection for cell and tissue lysates.

This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale.

Highlights:

  • Complete protection – all-in-one cocktail contains both protease and phosphatase inhibitors
  • Multiple package sizes – choose from four convenient packages sizes to match nearly any scale
  • Convenient – the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
  • Easy storage – aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
  • Compatible – can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
  • Validated – fully compatible with Thermo Scientific Pierce Cell Lysis Buffers

Product Details:

This combined cocktail is the first of its kind. The aqueous-based format allows for convenient cold-room storage without freezing, which is associated with DMSO-based cocktails, and is much easier to use than combining individual inhibitors or using other formats. More importantly, the cocktail formulation is more effective than competing products for inhibiting proteases in lysates and preserving protein phosphorylation during cell harvest. The formulation is also MS-compatible because it does not contain AEBSF, which can cause peaks to shift.

Protease and phosphatase inhibitors included in the cocktail formulation and their targeted enzyme class.
Inhibitor Target
Sodium Fluoride Ser/Thr and Acidic Phosphatases
Sodium Orthovanadate Tyr and Alkaline Phosphatases
β-glycerophosphate Ser/Thr Phosphatases
Sodium Pyrophosphate Ser/Thr Phosphatases
Aprotinin Ser Proteases
Bestatin Amino-peptidases
E64 Cysteine Proteases
Leupeptin Ser/Cys Proteases
EDTA (optional) Metalloproteases

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktails.
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktails are more effective than Roche protease inhibitor tablets. Using a general protease assay with a rat pancreas extract (1mg/mL), the Halt Protease and Phosphatase Inhibitor Cocktails (± EDTA) were compared with the tablet-based protease inhibitor cocktails (± EDTA) of Competitor Tablet. The same experimental conditions were used for all samples tested. A 1X final concentration of each inhibitor cocktail was added to the extract. The Halt Protease and Phosphatase Inhibitor Cocktails resulted in ≥ 97% inhibition compared to ≥ 62% inhibition for the tablets.

Performance comparison of commercial protease and phosphatase inhibitor cocktails and tablets.
Performance comparison of commercial protease and phosphatase inhibitor cocktails and tablets. HeLa cells were serum-starved for 24 hours. Serum-containing media was added to the cells for 15 minutes and cells were lysed with Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78501) in the presence or absence of 1X inhibitor cocktail. Tablets were dissolved in 1mL of buffer and then diluted 1:100 into the lysis reagent. Respective protease and phosphatase inhibitors were added directly to the lysis reagent. Lysates were analyzed by Western blot for preservation of protein phosphorylation using the antibodies indicated. Panel A. Phosphorylated PTEN, Panel B. Phosphorylated CDC2. The antibodies recognize phosphorylation on serine and tyrosine, respectively.

Related Resources:

Review of protease and phosphatase inhibitors

Related Products:

All Protease and Phosphatase Inhibitor Cocktails and Tablets


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
78440 Halt Protease and Phosphatase Inhibitor Cocktail (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 100mL of sample
1mL Product Instructions for product #78440 Halt Protease and Phosphatase Inhibitor Cocktail (100X) MSDS for product #78440 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Certificate of Analysis for product #78440 Halt Protease and Phosphatase Inhibitor Cocktail (100X) $214.00

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78442 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 240mL of sample in 10mL increments
24 x 100µL Product Instructions for product #78442 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) MSDS for product #78442 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) Certificate of Analysis for product #78442 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) $242.00

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78444 Halt Protease and Phosphatase Inhibitor Cocktail (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 500mL of sample
5 x 1mL Product Instructions for product #78444 Halt Protease and Phosphatase Inhibitor Cocktail (100X) MSDS for product #78444 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Certificate of Analysis for product #78444 Halt Protease and Phosphatase Inhibitor Cocktail (100X) $593.00

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78446 Halt Protease and Phosphatase Inhibitor Cocktail (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 1L of sample
10mL Product Instructions for product #78446 Halt Protease and Phosphatase Inhibitor Cocktail (100X) MSDS for product #78446 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Certificate of Analysis for product #78446 Halt Protease and Phosphatase Inhibitor Cocktail (100X) $878.00

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78441 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 100mL of sample
1mL Product Instructions for product #78441 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) MSDS for product #78441 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Certificate of Analysis for product #78441 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) $198.00

Add to cart
78443 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail, EDTA-Free (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 240mL of sample in 10mL increments
24 x 100µL Product Instructions for product #78443 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail, EDTA-Free (100X) MSDS for product #78443 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail, EDTA-Free (100X) Certificate of Analysis for product #78443 Halt Protease and Phosphatase Inhibitor Single-Use Cocktail, EDTA-Free (100X) $228.00

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78445 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 500mL of sample
5 x 1mL Product Instructions for product #78445 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) MSDS for product #78445 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Certificate of Analysis for product #78445 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) $575.00

Add to cart
78447 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 1L of sample
10mL Product Instructions for product #78447 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) MSDS for product #78447 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Certificate of Analysis for product #78447 Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) $860.00

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78441B Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Formulation: Inhibitors stabilized in aqueous solution
Custom MSDS for product #78441B Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Certificate of Analysis for product #78441B Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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