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Halt Phosphatase Inhibitor Cocktail


Safeguards samples against serine, threonine and protein tyrosine phosphatase activities.

Thermo Scientific Halt Phosphatase Inhibitor Cocktail

Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.

The Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases (PTPs) during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays. Furthermore, the cocktail is compatible with popular protein assays such as the Thermo Scientific Pierce BCA Protein Assay Kit (Part No. 23225) and Bradford-based Pierce Coomassie Plus Assay Kit (Part No. 23236).

Highlights:

  • All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
  • Compatible – works with standard protein assays, including BCA and coomassie (Bradford) assays
  • Convenient storage – refrigerator (2 to 8°C) storage saves valuable space in the freezer and provides for immediate use
  • Multiple sizes – select a package size of the 100X cocktail that is ideal for any particular scale and workflow of protein extraction experiment

Product Details:

Preserved phosphorylation states of three proteins in cell lysate.

Preserved phosphorylation states of three proteins in cell lysate. HeLa cells were lysed in the absence (-) and presence (+) of a 1X concentration of the Thermo Scientific Halt Phosphatase Inhibitor Cocktail. Cell lysates were analyzed by Western blot for total and phosphorylated protein as indicated.

Panel A: MEK and phosphorylated MEK (PMEK).

Panel B: MAP Kinase and PMAP Kinase.

Panel C: STAT3 and PSTAT3.

The proteins are phosphorylated on serine, threonine/tyrosine and tyrosine, respectively.

Preserved phosphorylation states of three proteins in cell lysate.
Preserved phosphorylation states of three proteins in cell lysate.

Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis. Halt Phosphatase Inhibitor Cocktail accomplishes this task.

The Halt Phosphatase Inhibitor Cocktail does not contain protease inhibitors. If necessary, protease inhibitors can be added along with the phosphatase inhibitor cocktail. Halt Phosphatase Inhibitor Cocktail is compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. Where incompatibilities arise, the components can be removed by dialysis or desalting before performing these incompatible procedures.

Halt Phosphatase Inhibitor is...

  • Devoid of protease inhibitors. To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail.
  • Dialyzable. Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components.
  • Supplied as a 100X concentrate that is effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X.
  • Compatible with all Pierce Cell Lysis Solutions.

Related Resources:

Review of protease and phosphatase inhibitors

Related Products:

All Protease and Phosphatase Inhibitor Cocktails and Tablets
Pierce Cell Lysis Solutions


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
78428 Halt Phosphatase Inhibitor Single-Use Cocktail
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 240mL of sample in 10mL increments
24 x 100┬ÁL Product Instructions for product #78428 Halt Phosphatase Inhibitor Single-Use Cocktail MSDS for product #78428 Halt Phosphatase Inhibitor Single-Use Cocktail Certificate of Analysis for product #78428 Halt Phosphatase Inhibitor Single-Use Cocktail $270.00

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78420 Halt Phosphatase Inhibitor Cocktail
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 100mL of sample
1mL Product Instructions for product #78420 Halt Phosphatase Inhibitor Cocktail MSDS for product #78420 Halt Phosphatase Inhibitor Cocktail Certificate of Analysis for product #78420 Halt Phosphatase Inhibitor Cocktail $176.00

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78420PROMO Halt Phosphatase Inhibitor Cocktail
Formulation: 3 x Part No. 78420

Promotional item; expires June 30, 2014
3 x 1mL $352.00

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78426 Halt Phosphatase Inhibitor Cocktail
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 500mL of sample
5 x 1mL Product Instructions for product #78426 Halt Phosphatase Inhibitor Cocktail MSDS for product #78426 Halt Phosphatase Inhibitor Cocktail Certificate of Analysis for product #78426 Halt Phosphatase Inhibitor Cocktail $535.00

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78427 Halt Phosphatase Inhibitor Cocktail
Formulation: Inhibitors stabilized in aqueous solution
Sufficient For: 1L of sample
10mL Product Instructions for product #78427 Halt Phosphatase Inhibitor Cocktail MSDS for product #78427 Halt Phosphatase Inhibitor Cocktail Certificate of Analysis for product #78427 Halt Phosphatase Inhibitor Cocktail $730.00

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78420B Halt Phosphatase Inhibitor Cocktail
Formulation: Inhibitors stabilized in aqueous solution
Custom MSDS for product #78420B Halt Phosphatase Inhibitor Cocktail Certificate of Analysis for product #78420B Halt Phosphatase Inhibitor Cocktail

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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