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Guanidine-HCl and 8M Solution


A denaturant with many protein research applications.

Pierce 8M Guanidine-HCl Solution

Thermo Scientific Pierce 8M Guanidine-HCl Solution is an accurately formulated, ready-to-use solution of guanidine hydrochloride, that can be easily diluted and pH-adjusted to any concentration below 8M.

In addition to increasing solubility of hydrophobic molecules, guanidine is a general protein denaturant, unfolding proteins and altering their three-dimensional structure. Consequently, some proteins will be irreversibly altered upon interaction with guanidine solutions and may lose their binding function. Before any large-scale use of guanidine, it is best to test a small sample and determine whether the denaturing effects will adversely affect the intended use of the protein.

Highlights (of 8M Guanidine-HCl Solution):

  • Any concentration below 8M guanidine can be quickly and easily prepared.
  • No wasting valuable research time weighing and dissolving guanidine crystals
  • Free from UV absorbing materials in the range of 225-300nm
  • Free from heavy metal contaminants
  • Free from degradation products such as ammonia
  • Excellent stability
    Guanidine-HCl

Applications for Guanidine Hydrochloride:

  • Solubilizing proteins from inclusion bodies
  • Increasing solubility of hydrophobic peptides and proteins
  • Denaturing proteins

Product Details:

Chaotropes, substances that disrupt the structure of water interactions, help to solubilize hydrophobic proteins and peptides for a variety of biological purposes. For example, hydrophobic peptides and proteins may be dissolved in coupling buffer containing either 6 molar guanidine-HCl or 4 molar urea prior to immobilization on AminoLink Plus Support to create an affinity column. However, because the degradation products of guanidine and urea include ammonia, which contains a primary amine that will compete for reaction to the support, urea and guanidine solutions must be prepared with high quality reagents immediately before use.

Properties of Guanidine Hydrochloride.
Alternative names guanidinium hydrochloride, guanidine Monohydrochloride, guanidine chloride, iminourea hydrochloride, carbamidine hydrochloride, aminomethanamidine
Molecular formula CN3H5 HCl
Molecular weight 95.54
CAS number 50-01-1

 

Specifications. Pierce Guanidine-Hydrochloride is manufactured to the highest specifications to ensure the integrity of your protein sample. Each lot of Guanidine-HCl is tested to meet the following minimum specifications.
Guanidine HCl (Part No. 24110)
Purity > 99.5%
UV analysis A280 < 0.010
(6 M solution) A260 < 0.060
Copper (Cu) <1 ppm
Iron (Fe) <1 ppm
Lead (Pb) <1 ppm
Zinc (Zn) <1 ppm
8M Guanidine HCl Solution (Part No. 24115)
Density 1.183 - 1.188
UV analysis A280 < 0.02; A260 < 0.03
UV cutoff 219 + 2nm

 

Guanidine Hydrochloride Molarity Table. Beginning with 10mL of 8M Guanidine Hydrochloride Solution, dilution to the indicated final volume will result in the stated molarity.
Molarity   Final  Volume   Molarity  Final Volume
8M  10mL   3M  26.7mL
7M  11.4mL   2M  40mL
6M  13.3mL   1.5M  52mL
5M  16mL   1M  80mL
4M  20mL   0.5M  160mL

References:

  1. Tanaka, S., et al. (1985) J. Biochem.97, 1377-1384.
  2. Wong, K.P., et al. (1971) J. Biochem.40, 459-464.

Related Products:

Urea
Inclusion Body Solubilization Reagent
Protein Refolding Kit


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
24110 Guanidine-HCl
Formulation: Pure (at least 99.5%) granular crystals of guanidine hydrochloride
Sufficient For: Preparing 661mL of 8M solution
500g MSDS for product #24110 Guanidine-HCl Certificate of Analysis for product #24110 Guanidine-HCl $132.00

Add to cart
24110B Guanidine-HCl
Formulation: Pure (at least 99.5%) granular crystals of guanidine hydrochloride
Custom MSDS for product #24110B Guanidine-HCl Certificate of Analysis for product #24110B Guanidine-HCl
24115 8M Guanidine-HCl Solution
Formulation: 8M Guanidine hydrochloride in ultrapure water
Sufficient For: Direct use or dilution
200mL MSDS for product #24115 8M Guanidine-HCl Solution Certificate of Analysis for product #24115 8M Guanidine-HCl Solution $180.00

Add to cart
24115B 8M Guanidine-HCl Solution
Formulation: 8M Guanidine hydrochloride in ultrapure water
Sufficient For: Direct use or dilution
Custom MSDS for product #24115B 8M Guanidine-HCl Solution Certificate of Analysis for product #24115B 8M Guanidine-HCl Solution

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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