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Immobilized Glutathione S-Transferase


Isolate or remove GST-specific antibodies.

Thermo Scientific Immobilized GSTThermo Scientific Immobilized GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilized on crosslinked, 6% beaded agarose.

Antibody molecules recognizing the GST portion of a recombinant fusion protein can be removed from the desired antibody by passing antiserum over an immobilized GST column. GST antibodies will bind to the immobilized GST column, whereas antibodies against other fusion proteins will pass through the column unrestrained. The expression of foreign proteins in E. coli using a glutathione S-transferase (GST) fusion system is a method of choice for studying the function of particular proteins, for purifying interacting factors and for generating antibodies against fused proteins.

Highlights:

  • Provides purification of specific antibodies from those directed against
    the GST tag
  • Reusable - bound antibodies can be eluted with 6 M guanidinium
    chloride allowing for 10 purifications with the support

References:

  1. Smith, D.B. and Johnson, K.S. (1988). Gene 67, 31-40.
  2. Bar-Peled, M. and Raikhel, N.V. (1996). Anal. Biochem. 241, 140-142.

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20205 Pierce GST Agarose Columns, 2mL
Formulation: 8mL-capacity columns containing 2mL of resin
Sufficient For: Binding approx. 1mg anti-GST antibody per column
2 columns Product Instructions for product #20205 Pierce GST Agarose Columns, 2mL MSDS for product #20205 Pierce GST Agarose Columns, 2mL Certificate of Analysis for product #20205 Pierce GST Agarose Columns, 2mL $436.00

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20211 Pierce GST Agarose
Formulation: Glutathione S-transferase on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding approx. 0.5mg anti-GST antibody per mL of resin
5mL Product Instructions for product #20211 Pierce GST Agarose MSDS for product #20211 Pierce GST Agarose Certificate of Analysis for product #20211 Pierce GST Agarose $470.00

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20237 Pierce GST
Formulation: Solution of glutathione S-transferase
Sufficient For: Activity ≥ 100 units per mg
1mg MSDS for product #20237 Pierce GST Certificate of Analysis for product #20237 Pierce GST $210.00

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20239 Pierce GST
Formulation: Solution of glutathione S-transferase
Sufficient For: Activity ≥ 100 units per mg
10 x 1mg $1,010.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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