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GSK3 alpha-beta In-Cell ELISA Kits


Microplate assays to measure total and phospho-GSK3 in whole cells.

Pierce GSK3 alpha/beta In-Cell ELISA Kits

Thermo Scientific Pierce GSK3 alpha/beta In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure total and phosphorylated glycogen synthase kinase 3 (phospho-GSK3) in whole cells.

These ELISA kits for whole cells include a mouse monoclonal anti-GSK3α/β antibody, rabbit monoclonal anti-phospho-(Ser21/Ser9) GSK3α/β antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. The GSK3α/β In-Cell ELISA Kits enable simultaneous detection of both total and phospho-(Ser21/Ser9) GSK3α/β.

Highlights:

  • Measures total and phosphorylated glycogen synthase kinase 3 (GSK3) in whole cells using a 96- or 384-well microplates
  • Two reliable detection formats available:
    • colorimetric detection kit for use with standard microplate readers
    • near infrared fluorescent detection kit for use with IR imaging system
  • Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
  • Provides better data and higher throughput than quantitative Western blotting
  • Less costly (one well vs. one lane) than Western blotting

Related Resources:

Overview and Menu of Target-Specific In-Cell ELISA Kits
Dylight Fluor Spectral Properties (Ex/Em)

Related Products:

ELISA reagent reservoirs and sealing tape
Secondary Antibodies and Conjugates For ELISAs and Western Blotting
DyLight Fluor Conjugated Secondary Antibodies and Biotin Binding Proteins

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62217 GSK3 alpha/beta Colorimetric In-Cell ELISA Kit
Formulation: Multi-component kit
Sufficient For: 96 microplate wells of overnight-cultured cells

Kit Contents:
Blocking Buffer, 50mL
20X Tris Buffered Saline, 50mL
Surfact-Amps 20 (10% Detergent), 10mL
Surfact-Amps X-100 (10% Detergent), 10mL
HRP Conjugate, 0.14mL
TMB Substrate, 58mL
TMB Stop Solution, 55mL
Janus Green Whole-Cell Stain, 50mL
Elution Buffer, 2 x 30mL
Thin Plate Seal Assembly, 8 each
Anti-Phospho GSK3 alpha/beta (S21/9) Antibody
Anti-GSK3 Antibody
96-test kit Product Instructions for product #62217 GSK3 alpha/beta Colorimetric In-Cell ELISA Kit MSDS for product #62217 GSK3 alpha/beta Colorimetric In-Cell ELISA Kit $380.00

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62222 GSK3 alpha/beta Near Infrared In-Cell ELISA Kit
Formulation: Multi-component kit
Sufficient For: 96 microplate wells of overnight-cultured cells

Kit Contents:
Blocking Buffer, 100mL
20X Tris Buffered Saline, 50mL
Surfact-Amps 20 (10% Detergent), 10mL
Surfact-Amps X-100 (10% Detergent), 10mL
DyLight 680 Goat Anti-Mouse IgG (H+L), 55µL
DyLight 800 Goat Anti-Rabbit IgG (H+L), 55µL
Thin Plate Seal Assembly, 8 each
Anti-Phospho GSK3 alpha/beta (S21/9) Antibody
Anti-GSK3 Antibody
96-test kit Product Instructions for product #62222 GSK3 alpha/beta Near Infrared In-Cell ELISA Kit MSDS for product #62222 GSK3 alpha/beta Near Infrared In-Cell ELISA Kit $380.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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