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Green Renilla Luciferase Reporter Assay Vectors


Renilla reporter plasmids to clone genes for luciferase assays.

Thermo Scientific pMCS-Green Renilla Luciferase Vector

The Thermo Scientific pMCS-Renilla Luc Vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.

The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Product Details:

The pMCS-, pCMV- and pTK-Green-Renilla Luc Vectors encode the Green Renilla luciferase reporter with excellent light intensity.

Map of pMCS-Green-Renilla Luc Vector for luciferase assays
Plasmid map of the Thermo Scientific pMCS-Green-Renilla Luc Vector. See the following downloadable files for complete vector and cloning site sequences.

 

Download the complete sequence (.txt) and printable data sheet (.pdf) of each vector.
Vector Vector Information Files
pMCS-Green Renilla Luc pMCS-Green Renilla Luc Sequence (.txt) Data Sheet (.pdf)
pCMV-Green Renilla Luc pCMV-Green Renilla Luc Sequence (.txt) Data Sheet (.pdf)
pTK-Green Renilla Luc pTK-Green Renilla Luc Sequence (.txt) Data Sheet (.pdf)

These vectors are subject to a limited use label license.

More Product Data

General References:

  1. Lorenz, W.W., et al. (1991). Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc. Natl. Acad. Sci. USA, 88:4438-4442.

Related Resources:

Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide

Related Products:

Renilla Flash, Glow and Dual Luciferase Assay Kits
All Pierce Luciferase Assay Kits and Vectors
Custom Reporter Vector Cloning Service


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
16152 pMCS-Green Renilla Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16152 pMCS-Green Renilla Luc Certificate of Analysis for product #16152 pMCS-Green Renilla Luc $57.00

Add to cart
16153 pCMV-Green Renilla Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16153 pCMV-Green Renilla Luc Certificate of Analysis for product #16153 pCMV-Green Renilla Luc $57.00

Add to cart
16154 pTK-Green Renilla Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16154 pTK-Green Renilla Luc Certificate of Analysis for product #16154 pTK-Green Renilla Luc $57.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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