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An amine-to-sulfhydryl crosslinker with a short spacer arm (7.3 angstroms).

Pierce GMBS

Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).


  • Reactive groups: NHS ester and maleimide
  • Reactive toward: amino and sulfhydryl groups
  • C4-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
  • NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
  • Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-GMBS
  • Short aliphatic spacer has low potential for eliciting an immune response

Product Details:

N-gamma-Maleimidobutyryl-oxysuccinimide ester; GMBS
Chemical structure of GMBS crosslinking reagent.


  1. Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
  2. Fujiwara, K., et al. (1988). J. Immunol. Meth. 112, 77-83.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22309 GMBS
Formulation: N-gamma-Maleimidobutyryl-oxysuccinimide ester; powder solid
Sufficient For: 178mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22309 GMBS MSDS for product #22309 GMBS Certificate of Analysis for product #22309 GMBS $135.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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