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Pierce Glycoprotein Staining Kit


For specific detection of glycoproteins on gels or membranes.

Pierce Glycoprotein Staining Kit for the in-gel detection of glycoproteins. Formerly GelCode Glycoprotein Staining Kit.

The Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

  • Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
  • Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
  • Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
  • Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16µg horseradish peroxidase per band
  • Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).
  • Gels or membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes

Includes:

  • Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)

Product Details:

Staining protocol for in-gel detection of glycoproteins using the Pierce Glycoprotein Staining Kit.
 
Protocol summary for the Thermo Scientific Pierce Glycoprotein Staining Kit.

To use the Pierce Glycoprotein Staining Kit, simply treat the gel or membrane containing separated proteins treated with the provided periodate-based Oxidation Reagent. This treatment oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are reacted with the Pierce Glycoprotein Stain Reagent to form Schiff-base bonds that result in the production of magenta bands of the stain. Traditionally called the periodate-acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions.

GelCode Blue Total Protein Stain compared to Pierce Glycoprotein Stain for glycosylated proteins.
Sensitive, specific staining of glycosylated proteins with the Thermo Scientific Pierce Glycoprotein Staining Kit. Identical protein samples were electrophoresed in identical polyacrylamide gels and then stained with the Pierce Glycoprotein Staining Kit (left) or the Thermo Scientific GelCode Blue Stain Reagent for total protein detection (right). Only the 5 or 6 proteins containing different amounts of glycosylation are detected with the Glycoprotein Stain. The left lane in each panel is prestained MW marker.

References:

  1. Misenheimer, T.M., (2001). J. Biol. Chem. 276, 45882-45887.
  2. Pio, R., et al. (2001). J. Biol. Chem. 276, 12292-12300.

Related Resources:

Protein Gel Stains Selection Guide
Protein gel electrophoresis technical guide
Protein glycosylation technical guide

Related Products:

Immobilized ConA and WGA Glycoprotein Isolation Kits
Glycoprotein Carbohydrate Estimation Kit


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
24562 Pierce Glycoprotein Staining Kit
Formulation: Three 250mL-bottles of reagent (1 liquid, 2 dry), plus positive and negative control proteins (1mg each)
Sufficient For: 10 mini gels (SDS-PAGE)

Kit Contents:
Glycoprotein Stain, 250mL

    Shipped Separately:
Oxidation Reagent, 2.5g powder to make 250mL
Reduction Reagent, 1.25g powder to make 250mL
Positive Control (Horseradish Peroxidase), 1mg
Negative Control (Soybean Trypsin Inhibitor), 1mg
750mL kit Product Instructions for product #24562 Pierce Glycoprotein Staining Kit MSDS for product #24562 Pierce Glycoprotein Staining Kit Certificate of Analysis for product #24562 Pierce Glycoprotein Staining Kit $251.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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