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Glycoprotein Carbohydrate Estimation Kit


Measures glycosylation as the percent of total purified glycoprotein mass.

Pierce Glycoprotein Carbohydrate Estimation Kit

The Thermo Scientific Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (percent carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

  • Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
  • Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
  • Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols
  • Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Applications:

  • Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents
  • Characterize and classify purified protein fractions from an affinity procedure
  • Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Related Resources:

Glycoprotein Carbohydrate Estimation Kit FAQ
An overview of glycosylation and methods to analyze glycoproteins and glycopeptides
Protein Assay Selection Guide

Related Products:

Pierce Glycoprotein Staining Kit
Glycoprotein Isolation Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
23260 Glycoprotein Carbohydrate Estimation Kit
Formulation: Multi-component kit
Sufficient For: 60 tube assays or 250 microplate assays

Kit Contents:
Sodium meta-Periodate, 500mg
Pierce Glycoprotein Detection Reagent, 500mg
Glycoprotein Assay Buffer, 250mL
Glycoprotein Standards Set, 5-protein set
500mg kit Product Instructions for product #23260 Glycoprotein Carbohydrate Estimation Kit MSDS for product #23260 Glycoprotein Carbohydrate Estimation Kit Certificate of Analysis for product #23260 Glycoprotein Carbohydrate Estimation Kit $347.00

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23259 Glycoprotein Standards Set
Formulation: Multi-component kit
Sufficient For: Preparing standard curves for glycoprotein carbohydrate estimation

Kit Contents:
Lysozyme, lyophilized, 2.5mg
BSA, lyophilized, 2.5mg
Ovalbumin, lyophilized, 2.5mg
Apo-tranferrin, lyophilized, 2.5mg
Fetuin, lyophilized, 0.25mg
alpha-Acid Glycoprotein, 0.25mg
5-protein set MSDS for product #23259 Glycoprotein Standards Set Certificate of Analysis for product #23259 Glycoprotein Standards Set $220.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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