Update profile
    Change password
    Sign out

GlycoLink Coupling Catalyst


Optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

GlycoLink Coupling Catalyst

The Thermo Scientific GlycoLink Coupling Catalyst decreases reaction times and increases aldehyde-hydrazide coupling efficiency, resulting in greater than 90% coupling of glycoproteins in 4 hours.

The GlycoLink Coupling Catalyst is a two component kit composed of aniline and sodium acetate buffer. The catalyst accelerates the coupling rate of hydrazide and alkoxyamine moieties with reactive aldehydes that have been generated by periodate-oxidation of cis-diols in glycoproteins. The GlycoLink Coupling Catalyst increases the coupling efficiency of these reactions, decreasing the molar excess amounts of labeling reagents that are required for conjugation reactions. The catalyst is also useful for increasing the binding efficiency of oxidized glycoproteins or other carbohydrate-containing molecules to hydrazide resins.

Highlights:

  • Optimized – catalyzes aldehyde-hydrazide and aldehyde-alkoxyamine conjugation reaction
  • Efficient – maximizes coupling efficiency and decreases required coupling reaction incubation time
  • Gentle – does not interfere with protein function and is easily removed by desalting or dialysis

Applications:

  • Labeling aldehyde-containing molecules with alkoxyamine-biotins
  • Labeling aldehyde-containing molecules with hydrazide-biotins
  • Increase coupling efficiency of aldehyde-containing molecules to hydrazide resin

Product Details:

Producing reactive aldehydes via sodium meta-periodate oxidation of cis-diols in glycoproteins is a useful technique for variety of protein labeling and detection methods. The GlycoLink Coupling Catalyst (aniline) increases the efficiency of these reactions by acting as a nucleophilic catalyst that accelerates bond formation through a Schiff base intermediate. The catalyst also reduces the amount (molar excess) of hydrazide or alkoxyamine required in labeling reactions, thereby eliminating the need for DMSO to solubilize high concentrations of labeling reagent.

Glycoprotein coupling to immobilized hydrazide resin is increased using the GlycoLink Coupling Catalyst The Thermo Scientific GlycoLink Coupling Catalyst increases binding efficiency of glycoproteins to hydrazide resin. Human IgG was oxidized with 10mM sodium meta-periodate for 30 minutes. The oxidized IgG (10mg) was diluted to 5mg/mL either with GlycoLink Coupling Buffer alone or with GlycoLink Coupling Catalyst and coupled to 1mL of hydrazide resin. Results are the average of three separate conjugations for each condition after incubating for 4 hours.

More Product Data

General References:

  1. Abraham, R., et al. (1991). The influence of periodate oxidation on monoclonal antibody avidity and immunoreactivity. J. Immunol. Methods 144(1):77-86.
  2. Byeon, J.Y., et al. (2010). Efficient bioconjugation of protein capture agents to biosensor surfaces using aniline-catalyzed hydrazone ligation. Langmuir 26(19):15430-5.
  3. Dirksen, A., et al. (2006). Nucleophilic catalysis of hydrazone formation and transimination: implications for dynamic covalent chemistry. J. Am. Chem. Soc. 128(49):15602-3.
  4. Dirksen, A., Dawson, PE. (2008). Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling. Bioconjug. Chem. 19(12):2543-8.
  5. Domen, P., et al. (1990). Site-directed immobilization of proteins. J. Chromatogr. 510:293-302.
  6. O’Shannessy, D., et al. (1984). A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties. Immunol. Lett. 8:273-7.
  7. Zing, Y., et al. (2009). High-efficiency labeling of sialylated glycoproteins on living cells. Nat. Methods 6(3):207-9.

Related Resources:

GlycoLink FAQ
Review of glycosylation and methods to analyze glycoproteins and glycopeptides
Review of affinity purification
Review of covalent immobilization methods
Hydrazide crosslinker chemistry

Related Products:

GlycoLink Immobilization Kits and Resins
GlycoLink Immunoprecipitation Kit
Sodium meta-Periodate
Hydrazide- and Alkoxyamine-activated Biotinylation Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88944 GlycoLink Coupling Catalyst
Formulation: Two-component kit with catalyst and equilibration buffer
Sufficient For: 22mL of typical 1X reaction solution


Kit Contents:
GlycoLink Coupling Buffer, 100mL
Aniline, 0.2mL
100mL-kit Product Instructions for product #88944 GlycoLink Coupling Catalyst MSDS for product #88944 GlycoLink Coupling Catalyst Certificate of Analysis for product #88944 GlycoLink Coupling Catalyst $55.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
46430 - Restore PLUS Western Blot Stripping Buffer

34028 - 1-Step Ultra TMB-ELISA Substrate Solution

87748 - Protein Concentrators, 9K MWCO, 7mL

66380 - Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747