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Pierce Glutathione Magnetic Beads


Superior GST-binding particles for automated magnetic separations.

Thermo Scientific Pierce Magnetic Glutathione Beads for affinity purification of GST tagged fusion proteins.

Thermo Scientific Pierce Glutathione Magnetic Beads provide a simple, rapid and reliable method to purify glutathione S-transferase (GST) fusion proteins from crude cell lysate prepared from bacteria, yeast or mammalian cells.

The high-performance, iron oxide, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

Learn more about automated magnetic separations and Thermo Scientific Automated Solutions.

Highlights:

  • High-performance beads – non-aggregating, silanized, iron oxide, paramagnetic microparticles provide exceptional uniformity for automated HTS applications
  • Stable affinity ligand – glutathione is covalently immobilized to particles, ensuring leach-resistance and clean purification products
  • High capacity – binding capacity is sufficient for both routine and demanding magnetic separation procedures

Product Details:

Characteristics of Thermo Scientific Pierce Glutathione Magnetic Beads.
Composition: Iron oxide particles with reduced glutathione attached to the surface and encapsulated in crosslinked agarose
Magnetization: Superparamagnetic (no magnetic memory)
Bead Diameter: 1 to 10µm
Concentration: 25% slurry (based on volume) in 20% ethanol
Binding Capacity: 5 to 10mg GST/mL of settled beads

Large figures require title and legend to be placed below.
High-performance purification of a GST fusion protein using Thermo Scientific Pierce Glutathione Magnetic Beads. Bacterial cells expressing GST-Rabaptin were lysed, and replicate aliquots were processed with the Glutathione Magnetic Beads in a 96 deep-well plate using a Thermo Scientific KingFisher 96 Instrument. Eluates were boiled in reducing sample buffer, resolved by SDS-PAGE and stained with coomassie dye. Purity and reproducibility were excellent.

Glutathione S-transferase (GST) is a protein (220 amino acids in length) that is commonly added to the end of recombinant proteins to aid in their purification or detection. Glutathione is composed of three amino acids (Glu-Cys-Gly) and is sometimes abbreviated as (GSH). To make an affinity support, glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding. Addition of reduced glutathione in a physiologic buffer is sufficient to competitively displace (elute) and recover the GST fusion protein from the immobilized glutathione.

Related Resources:

Review of magnetic separations for proteomics (includes selection guide)

Related Products:

MagnaBind Magnetic Beads for manual applications
Glutathione Agarose Resins, Columns and Kits
Thermo Scientific KingFisher Magnetic Particle Processors


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88821 Pierce Glutathione Magnetic Beads
Formulation: Glutathione on paramagnetic iron oxide particles (1 to 10µm dia.) slurried in 20% ethyl alcohol
Sufficient For: Binding 5 to 10mg GST per mL of beads
4mL Product Instructions for product #88821 Pierce Glutathione Magnetic Beads MSDS for product #88821 Pierce Glutathione Magnetic Beads Certificate of Analysis for product #88821 Pierce Glutathione Magnetic Beads $128.00

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88822 Pierce Glutathione Magnetic Beads
Formulation: Glutathione on paramagnetic iron oxide particles (1 to 10µm dia.) slurried in 20% ethyl alcohol
Sufficient For: Binding 5 to 10mg GST per mL of beads
20mL Product Instructions for product #88822 Pierce Glutathione Magnetic Beads MSDS for product #88822 Pierce Glutathione Magnetic Beads Certificate of Analysis for product #88822 Pierce Glutathione Magnetic Beads $465.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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