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Pierce Glutathione Coated Plates


Bind recombinantly expressed GST-fusion proteins for plate-based analysis.

Pierce Glutathione Coated Plates

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

Highlights:

  • Selective – specifically binds fusion proteins containing glutathione S-transferase
  • Capacity – binds approx. 10ng GST protein per well
  • Versatile – ready-to-bind plate format is useful for protein interaction studies
  • Compatible – detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
  • Ready to use – Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding
  • Three formats – available as clear, white or black 96-well microplates

Product Details:


Amount of GST applied to each well (ng)
 
Binding of GST to Thermo Scientific Pierce Glutathione Coated Plate.

References:

  1. Pullen, S.S., et al. (1999). J. Biol. Chem. 274, 14246-14254.
  2. McKevitt, M., et al. (2003). Genome Res. 13, 1665-1674.
  3. Yarwood, S.J., et al. (1999). J. Biol. Chem. 274, 14909-14917.

Related Resources:

Assay development and ELISA technical guide
GST-tagged proteins technical guide

Related Products:

Pierce Glutathione Agarose and GST Purification Kits
Anti-GST Antibody Coated Plates
B-PER Bacterial Protein Extraction Reagents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15140 Glutathione Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 100μL/well with proprietary GSH-presenting molecular solution and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 10ng GST-tagged protein per well

5 plates Product Instructions for product #15140 Glutathione Coated Plates, Clear, 8-Well Strip MSDS for product #15140 Glutathione Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15140 Glutathione Coated Plates, Clear, 8-Well Strip $172.00

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15140B Glutathione Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 100µL/well with proprietary GSH-presenting molecular solution and blocked at 200µL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 10ng GST-tagged protein per well
Custom MSDS for product #15140B Glutathione Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15140B Glutathione Coated Plates, Clear, 8-Well Strip $5,000.00

15240 Glutathione Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 100μL/well with proprietary GSH-presenting molecular solution and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 10ng GST-tagged protein per well

5 plates Product Instructions for product #15240 Glutathione Coated Plates, White, 96-Well MSDS for product #15240 Glutathione Coated Plates, White, 96-Well Certificate of Analysis for product #15240 Glutathione Coated Plates, White, 96-Well $172.00

Add to cart
15340 Glutathione Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 100μL/well with proprietary GSH-presenting molecular solution and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 10ng GST-tagged protein per well

5 plates Product Instructions for product #15340 Glutathione Coated Plates, Black, 96-Well MSDS for product #15340 Glutathione Coated Plates, Black, 96-Well Certificate of Analysis for product #15340 Glutathione Coated Plates, Black, 96-Well $172.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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