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GelCode Blue Stain Reagent


A remarkable and convenient coomassie stain for protein gels (SDS-PAGE).

GelCode Blue Stain, a coomassie dye protein gel stain

Thermo Scientific GelCode Blue Stain Reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection and excellent clarity for polyacrylamide gels.

GelCode Blue Stain Reagent uses the colloidal properties of coomassie G-250 dye for polyacrylamide gel protein staining. This unique reagent stains only protein and, unlike traditional coomassie stains based on the R-250 form of the dye, allows bands to be viewed directly on the gel during the staining process. After staining, an optional water-wash step further enhances staining sensitivity and yields a clear background. With GelCode Blue Stain there is no need for multistep destaining procedures typically associated with other gel staining systems.

Highlights:

  • No methanol-acetic acid destaining required – saves time, reduces reagent costs and minimizes solvent disposal
  • Fast, one-step, one-hour staining – ready-to-use stain reagent; band development is visible directly in the staining tray; no background from overnight staining; flexible fixing and washing protocol; no gel shrinkage; stained gel can be dried
  • More sensitive than standard coomassie gel stains – some protein bands visible to 8ng; wide linear range for densitometric analysis (ref 1); allows gel staining after Western transfer; compatible with MALDI-TOF analysis and sequence analysis (refs 2-5)
  • Water wash increases staining sensitivity – optional step provides crystal-clear gel background and makes weakly stained bands more visible

Product Details:

GelCode Blue coomassie G-250-based gel staining protocol
 
Protocol summary for the Thermo Scientific GelCode Blue Stain Reagent.

This unique GelCode Blue Stain Reagent stains only protein and allows bands to be viewed directly in the gel during the 1 hour gel staining process. After staining, a water equilibration step (Water Wash Enhancement) further enhances staining sensitivity and yields a clear gel background. Because the destaining step does not fix the protein, GelCode Blue Stain is compatible with mass spectrometry analysis and N-terminal sequence analysis.

Traditional Coomassie Stain:    GelCode Blue Stain Reagent:
Traditional Coomassie Stain    Coomassie G-250-based GelCode Blue Stain Reagent
Staining time: 1 hour
No bands visible before destaining.
   Staining time: 1 hour
Bands visible in the staining tray.
destaining coomassie gel with acetic acid    Water destaining with Coomassie G-250-based GelCode Blue Stain Reagent
After destaining: 1 hour
in methanol:acetic acid.
 
   After destaining: 1 hour
Water Wash Enhancement.
 
Comparison of a traditional homemade coomassie gel stain reagent and the Thermo Scientific GelCode Blue Stain Reagent.

GelCode Blue Stain Reagent contains additives that help to slow down the formation of dye-dye and dye-protein aggregates, which form in all coomassie dye-based protein staining reagents. Nevertheless, if left undisturbed, the reagent will form visible dye-dye aggregates that settle in the bottom of the bottle. Fortunately, gently inverting or tipping-and-swirling the bottle several times completely disperses these aggregates. Therefore, it is good practice to mix the stain reagent before pouring or dispensing to ensure that a homogeneous sample of the reagent is used.

References:

  1. Mateer, S.C., et al. (2002). J. Biol. Chem. 227, 12324-12333.
  2. Aulak, K.S., et al. (2001). Proc. Natl. Acad. Sci. USA 98, 12056-12061.
  3. Lim, J., et al. (2002). J. Biol. Chem. 277, 20774-20782.
  4. Hilton, J.M., et al. (2001). J. Biol. Chem. 276, 16341-16347.
  5. Tani, M., et al. (2002). J. Biol. Chem. 275, 3462-3468.

Related Resources:

GelCode Blue Stain Reagent FAQ
Protein Gel Stains Selection Guide
Protein gel electrophoresis technical guide
Process stained polyacrylamide gel pieces for mass spectrometry (Tech Tip #50)
Purify proteins from polyacrylamide gels (Tech Tip #51)

Related Products:

GelCode Blue Safe Stain Reagent
Imperial Protein Stain
Coomassie Brilliant Blue R-250 and G-250 Dyes
Reversible Protein Stain for Membranes


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
24590 GelCode Blue Stain Reagent
Formulation: Proprietary acidic solution of coomassie blue G-250 dye
Sufficient For: 20 to 25 mini gels (SDS-PAGE)
500mL Product Instructions for product #24590 GelCode Blue Stain Reagent MSDS for product #24590 GelCode Blue Stain Reagent Certificate of Analysis for product #24590 GelCode Blue Stain Reagent $77.00

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24592 GelCode Blue Stain Reagent
Formulation: Proprietary acidic solution of coomassie blue G-250 dye
Sufficient For: 140 to 175 mini gels (SDS-PAGE)
3.5L Product Instructions for product #24592 GelCode Blue Stain Reagent MSDS for product #24592 GelCode Blue Stain Reagent Certificate of Analysis for product #24592 GelCode Blue Stain Reagent $267.00

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72300 Pump (for 3.5 liter package only)
A complementary reagent dispensing pump attachment is available FREE upon request for Product # 24592. Specify Product # 72300 when you place your order.
1 pump $0.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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