Thermo Scientific GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping costs.
GelCode Blue Safe Protein Stain is a coomassie G-250 dye-based reagent for staining protein on polyacrylamide gels and membranes. GelCode Blue Safe Stain provides considerable sensitivity, allowing for the detection down to 9ng of protein. This protein-specific stain allows bands to be viewed directly on a gel or membrane during staining and is compatible with mass spectrometry applications. The staining process is simple and flexible, usually requiring a 15-minute incubation of the gel or sample with the GelCode Blue Safe Protein Stain, followed by a simple water wash to yield blue protein bands and a clear background.
Because the supplied Activator Crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements.
- Sensitive and fast – detect down to 9ng of protein per band in 15 minutes
- Safe – non-corrosive to skin, non-flammable and safe to ship and store until activated
- Convenient – water washes only; no acid-fixative or methanol destain solutions required
- Versatile – suitable for staining 1D and 2D polyacrylamide gels, nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
- Compatible – useful for qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
- Easy to use – add and dissolve activator crystals before first use, then simply add reagent to stain gels
- Stable – store non-activated or activated stain at room temperature for up to one year
|Protocol summary for the Thermo Scientific GelCode Blue Safe Stain.
For proper protein staining, the Activator Crystals must be dissolved in the staining reagent before use. Add the entire contents of the Activator Crystals bottle into the GelCode Blue Safe Protein Stain bottle. Dissolve the crystals by mixing end-over-end for 1 minute, and then allow it to remain at room temperature for 5 minutes before use. Store the activated stain at room temperature.
|Thermo Scientific GelCode Blue Safe Protein Stain is fast and sensitive. A mixture of proteins (myosin, β-galactosidase, phosphorylase B, BSA, ovalbumin, carbonic anhydrase, soybean trypsin inhibitor, lysozyme and aprotinin) was electrophoresed on 4-20% Precise Protein Gels (Part No. 25224). The gels were stained with GelCode Blue Safe Protein Stain for five minutes, 15 minutes and one hour. All gels were destained for one hour in ultrapure water. Lane 1-8: Proteins at 1000ng, 500ng, 250ng, 125ng, 63ng, 31ng, 16ng and 8ng.
Quantitative densitometry performance of protein stains. Varying concentrations of BSA (2ng to 1000ng) were electrophoresed on 4-20% Precise Protein Gels (Part No. 25224). Gels were washed in water (3 x 5 min) and stained with Thermo Scientific GelCode Blue Safe Protein Stain or stains from other suppliers for one hour and destained with water for 1 hour. Densitometry values were obtained using Kodak MI™ software.
||R2 Value for linear range
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