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Gaussia Luciferase Reporter Assay Vectors


Plasmids to clone genes to express proteins for secreted luciferase assays.

Thermo Scientific pMCS-Gaussia Luc Vector

The Thermo Scientific pMCS-Gaussia Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter.

The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Product Details:

The pMCS-, pCMV- and pTK-Gaussia Luc Vectors encode the Gaussia luciferase reporter with excellent light intensity.

Map of pMCS-Gaussia Luc Vector for luciferase assays
Plasmid map of the Thermo Scientific pMCS-Gaussia Luc Vector. See the following downloadable files for complete vector and cloning site sequences.

 

Download the complete sequence (.txt) and printable data sheet (.pdf) of each vector.
Vector Vector Information Files
pMCS-Gaussia Luc pMCS-Gaussia Luc Sequence (.txt) Data Sheet (.pdf)
pCMV-Gaussia Luc pCMV-Gaussia Luc Sequence (.txt) Data Sheet (.pdf)
pTK-Gaussia Luc pTK-Gaussia Luc Sequence (.txt) Data Sheet (.pdf)

These vectors are subject to a limited use label license.

More Product Data

General References:

  1. Tannous, B. A., et al. (2005). Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Molecular Therapy 11:435-443.

Product References:

  1. Varsano, T., et al. (2013). Inhibition of melanoma growth by small molecules that promote the mitochondrial localization of ATF2. Clin. Cancer Res. 19:2710-22.

Related Resources:

Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide

Related Products:

Gaussia Luciferase Flash Assay Kit
Gaussia-Firefly Dual Luciferase Flash Assay Kit
pMCS-Gaussia-Dura Luc Vector and Glow Assay Kit
All Pierce Luciferase Assay Kits and Vectors
Custom Reporter Vector Cloning Service


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
16146 pMCS-Gaussia Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16146 pMCS-Gaussia Luc Certificate of Analysis for product #16146 pMCS-Gaussia Luc $57.00

Add to cart
16147 pCMV-Gaussia Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16147 pCMV-Gaussia Luc Certificate of Analysis for product #16147 pCMV-Gaussia Luc $57.00

Add to cart
16148 pTK-Gaussia Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16148 pTK-Gaussia Luc Certificate of Analysis for product #16148 pTK-Gaussia Luc $57.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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