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Fast Western Blot Kits, SuperSignal West Pico


The increased Western blot sensitivity you need with speed you want.

Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate

Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

Highlights:

  • Fast – picogram levels of detection in about 1 hour
  • Convenient – no expensive hardware or vacuum required; no clogging issues
  • Simple – optimized protocol makes Western blot analysis easier than ever
  • Economical – cost as little as $14 per blot; no expensive consumables or extra equipment
  • Excellent stability – kit is stable for 1 year stored at 4°C
  • Easy – complete kits contain all components needed to block, probe and develop a blot with your mouse or rabbit primary antibody

Product Details:

The Pierce Fast Western Blot Kits are reagent-based system that provide optimized reagents for blocking, antibody dilution and detection of Western blots. The Fast Western Blot Kit protocol is quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other popular rapid Western blotting systems.


Detection of XIAP and TSC1 with SuperSignal West Pico Fast Western Blot Kit
 
Classic vs. fast Western protocol using Thermo Scientific SuperSignal West Pico Substrate. 293T cell lysate was diluted in reducing sample buffer and loaded to a gel. After electrophoresis, proteins were transferred to nitrocellulose and PVDF membranes using a semi-dry blotter for 10 minutes at 25V. Half of both membranes was developed using the classic Western protocol, and the other half was developed using the fast Western protocol. XIAP detection: The blots were probed with mouse anti-XIAP antibody (1µg/mL, BD Pharmingen), incubated in SuperSignal West Pico Substrate Working Solution for 5 minutes and exposed to film for 1 minute. TSC1 detection: The blots were probed with mouse anti-TSC1 antibody (1/1000 dilution, Cell Signaling Technology), incubated in SuperSignal West Pico Substrate Working Solution for 5 minutes and exposed to film for 5 minutes.

 

Comparision of common Western blotting protocols. Side by side comparison of the steps and times required to complete a Western blot (post transfer and before development with substrate) using a classical protocol, the reagent-based Thermo Scientific Pierce Fast Western System, and the instrument-based SNAP i.d.™ System (Millipore Corp).
Protocol Steps Classical protocol Pierce Fast System SNAP i.d.™ System
Block/Pretreat 60 min - - - -
Primary antibody 60 min 30 min 10 min
Wash 45 min - - 1 min
Secondary antibody 60 min 10 min 10 min
Wash 45 min 15 min 1 min
Total Time 270 min 55 min 22 min
Key limitations Preparation of fresh non-fat dry milk blocking buffer each time For use with mouse or rabbit primary antibodies Only 2 full-sized blots per cycle. Strips limited by the format of the blot holders available. Reliable vacuum source required

 

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate is a cost-effective means to obtain Western blot results quickly. This table provides a brief cost of reagents and required consumables used in a classical Western protocol, the Pierce Fast Western reagent-based system and Millipore SNAP i.d.™ system.
Western blot reagents Classical
Protocol
Pierce Fast
System
SNAP i.d.
System
Blot holder for instrument - - - - $7.33
Blotting membrane (NC) $2.48 $2.48 $2.48
Blocking buffer $0.02 - - $1.32
Wash buffer (TBST) $1.48 - - $1.48
Secondary antibody conjugate $0.15 - - $0.15
Detection substrate
(SuperSignal West Pico Substrate)
$4.30 - - $4.30
Film (5x7) $1.01 $1.01 $1.01
Kit's price per blot - - $9.95 - -
Total price per blot $9.44 $13.44 $18.07
Prices based on 2009 Thermo Scientific Pierce Western Blot Reagents and using SuperSignal West Pico Chemiluminescent Substrate as the detection reagent for comparable levels of sensitivity.

Related Products:

Pierce G2 Fast Blotter
Membranes and Filter Paper
CL-Xposure Film for Western blotting
Pierce Western Blot Signal Enhancer
Restore PLUS Western Blot Stripping Buffer
Browse all Pierce Western Blotting Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
35065 Fast Western Kit, SuperSignal West Pico, Mouse
Formulation: Multi-component kit
Sufficient For: 5 mini blots probed with mouse primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 100mL
Fast Western 10X Wash Buffer, 50mL
Fast Western Mouse Optimized HRP Reagent, Pico, 5mL
SuperSignal West Pico Luminol/Enhancer Solution, 25mL
SuperSignal West Stable Peroxide Solution, 25mL
200mL kit Product Instructions for product #35065 Fast Western Kit, SuperSignal West Pico, Mouse MSDS for product #35065 Fast Western Kit, SuperSignal West Pico, Mouse Certificate of Analysis for product #35065 Fast Western Kit, SuperSignal West Pico, Mouse $109.00

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35060 Fast Western Kit, SuperSignal West Pico, Mouse
Formulation: Multi-component kit
Sufficient For: 20 mini blots probed with mouse primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 400mL
Fast Western 10X Wash Buffer, 200mL
Fast Western Mouse Optimized HRP Reagent, Pico, 20mL
SuperSignal West Pico Luminol/Enhancer Solution, 100mL
SuperSignal West Stable Peroxide Solution, 100mL
800mL kit Product Instructions for product #35060 Fast Western Kit, SuperSignal West Pico, Mouse MSDS for product #35060 Fast Western Kit, SuperSignal West Pico, Mouse Certificate of Analysis for product #35060 Fast Western Kit, SuperSignal West Pico, Mouse $221.00

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35066 Fast Western Kit, SuperSignal West Pico, Rabbit
Formulation: Multi-component kit
Sufficient For: 5 mini blots probed with rabbit primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 100mL
Fast Western 10X Wash Buffer, 50mL
Fast Western Rabbit Optimized HRP Reagent, Pico, 5mL
SuperSignal West Pico Luminol/Enhancer Solution, 25mL
SuperSignal West Stable Peroxide Solution, 25mL
200mL kit Product Instructions for product #35066 Fast Western Kit, SuperSignal West Pico, Rabbit MSDS for product #35066 Fast Western Kit, SuperSignal West Pico, Rabbit Certificate of Analysis for product #35066 Fast Western Kit, SuperSignal West Pico, Rabbit $109.00

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35061 Fast Western Kit, SuperSignal West Pico, Rabbit
Formulation: Multi-component kit
Sufficient For: 20 mini blots probed with rabbit primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 400mL
Fast Western 10X Wash Buffer, 200mL
Fast Western Rabbit Optimized HRP Reagent, Pico, 20mL
SuperSignal West Pico Luminol/Enhancer Solution, 100mL
SuperSignal West Stable Peroxide Solution, 100mL
800mL kit Product Instructions for product #35061 Fast Western Kit, SuperSignal West Pico, Rabbit MSDS for product #35061 Fast Western Kit, SuperSignal West Pico, Rabbit Certificate of Analysis for product #35061 Fast Western Kit, SuperSignal West Pico, Rabbit $221.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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