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Fast Western Blot Kits, SuperSignal West Femto


The ultimate in Western blot sensitivity and speed.

Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate

Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate’s sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with either a mouse or rabbit primary antibody.

Highlights:

  • Fast – femtogram levels of detection in about 1 hour
  • Convenient – no expensive hardware or vacuum required; no clogging issues
  • Simple – optimized protocol makes Western blot analysis easier than ever
  • Easy – complete kits contain all components needed to block, probe and develop a blot with your mouse or rabbit primary antibody

Product Details:


Ezrin and Akt1 detected with SuperSignal West Femto Fast Western Blot Kit
 
Classic vs. fast Western protocol using Thermo Scientific SuperSignal West Femto Substrate. Cell lysate was diluted by 50% and separated by electrophoresis. Proteins were transferred to nitrocellulose and PVDF membranes using the a sem-dry blotter for 10 minutes at 25V. Membranes were developed using either the classic Western blotting protocol or the fast Western protocol. Each blot was incubated in SuperSignal West Femto Substrate Working Solution for 5 minutes and exposed to film. Ezrin detection: HeLa cell lysates were probed with mouse anti-ezrin antibody (1µg/mL, Lab Vision) and exposed to film for 1 minute. Akt1 detection: 293T cell lysates were probed with mouse anit-Akt1 antibody (1/2000 dilution, Cell Signaling Technology) and exposed to film for 30 seconds.

 

Comparision of common Western blotting protocols. Side by side comparison of the steps and times required to complete a Western blot (post transfer and before development with substrate) using a classical protocol, the reagent-based Thermo Scientific Pierce Fast Western System, and the instrument-based SNAP i.d.™ System (Millipore Corp).
Protocol Steps Classical protocol Pierce Fast System SNAP i.d.™ System
Block/Pretreat 60 min - - - -
Primary antibody 60 min 30 min 10 min
Wash 45 min - - 1 min
Secondary antibody 60 min 10 min 10 min
Wash 45 min 15 min 1 min
Total Time 270 min 55 min 22 min
Key limitations Preparation of fresh non-fat dry milk blocking buffer each time For use with mouse or rabbit primary antibodies Only 2 full-sized blots per cycle. Strips limited by the format of the blot holders available. Reliable vacuum source required

 

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate is a cost-effective means to obtain Western blot results quickly. This table provides a brief cost of reagents and required consumables used in a classical Western protocol, the Pierce Fast Western reagent-based system and Millipore SNAP i.d.™ system.
Western blot reagents Classical
Protocol
Pierce Fast
System
SNAP i.d.
System
Blot holder for instrument - - - - $7.33
Blotting membrane (NC) $2.48 $2.48 $2.48
Blocking buffer $0.02 - - $1.32
Wash buffer (TBST) $1.48 - - $1.48
Secondary antibody conjugate $0.15 - - $0.15
Detection substrate
(SuperSignal West Femto Substrate)
$26.65 - - $26.65
Film (5x7) $1.01 $1.01 $1.01
Kit's price per blot - - $35.44 - -
Total price per blot $31.83 $38.93 $40.46
Prices based on 2010 Thermo Scientific Pierce Western Blot Reagents and using SuperSignal West Femto Chemiluminescent Substrate as the detection reagent for comparable levels of sensitivity.

Related Products:

Pierce G2 Fast Blotter
Membranes and Filter Paper
CL-Xposure Film for Western blotting
Pierce Western Blot Signal Enhancer
Restore PLUS Western Blot Stripping Buffer
Browse all Pierce Western Blotting Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
35085 Fast Western Blot Kits, SuperSignal West Femto, Mouse
Formulation: Multi-component kit
Sufficient For: 2 mini blots probed with mouse primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 40mL
Fast Western 10X Wash Buffer, 20mL
Fast Western Mouse Optimized HRP Reagent, Femto, 2mL
SuperSignal West Femto Luminol/Enhancer Solution, 10mL
SuperSignal West Stable Peroxide Solution, 10mL
80mL kit Product Instructions for product #35085 Fast Western Blot Kits, SuperSignal West Femto, Mouse MSDS for product #35085 Fast Western Blot Kits, SuperSignal West Femto, Mouse Certificate of Analysis for product #35085 Fast Western Blot Kits, SuperSignal West Femto, Mouse $109.00

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35080 Fast Western Blot Kits, SuperSignal West Femto, Mouse
Formulation: Multi-component kit
Sufficient For: 10 mini blots probed with mouse primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 200mL
Fast Western 10X Wash Buffer, 100mL
Fast Western Mouse Optimized HRP Reagent, Femto, 10mL
SuperSignal West Femto Luminol/Enhancer Solution, 50mL
SuperSignal West Stable Peroxide Solution, 50mL
400mL kit Product Instructions for product #35080 Fast Western Blot Kits, SuperSignal West Femto, Mouse MSDS for product #35080 Fast Western Blot Kits, SuperSignal West Femto, Mouse Certificate of Analysis for product #35080 Fast Western Blot Kits, SuperSignal West Femto, Mouse $394.00

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35086 Fast Western Blot Kits, SuperSignal West Femto, Rabbit
Formulation: Multi-component kit
Sufficient For: 2 mini blots probed with rabbit primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 40mL
Fast Western 10X Wash Buffer, 20mL
Fast Western Rabbit Optimized HRP Reagent, Femto, 2mL
SuperSignal West Femto Luminol/Enhancer Solution, 10mL
SuperSignal West Stable Peroxide Solution, 10mL
80mL kit Product Instructions for product #35086 Fast Western Blot Kits, SuperSignal West Femto, Rabbit MSDS for product #35086 Fast Western Blot Kits, SuperSignal West Femto, Rabbit Certificate of Analysis for product #35086 Fast Western Blot Kits, SuperSignal West Femto, Rabbit $109.00

Add to cart
35081 Fast Western Blot Kits, SuperSignal West Femto, Rabbit
Formulation: Multi-component kit
Sufficient For: 10 mini blots probed with rabbit primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 200mL
Fast Western 10X Wash Buffer, 100mL
Fast Western Rabbit Optimized HRP Reagent, Femto, 10mL
SuperSignal West Femto Luminol/Enhancer Solution, 50mL
SuperSignal West Stable Peroxide Solution, 50mL
400mL kit Product Instructions for product #35081 Fast Western Blot Kits, SuperSignal West Femto, Rabbit MSDS for product #35081 Fast Western Blot Kits, SuperSignal West Femto, Rabbit Certificate of Analysis for product #35081 Fast Western Blot Kits, SuperSignal West Femto, Rabbit $394.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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