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Fast Western Blot Kits, SuperSignal West Dura


The increased Western blot sensitivity you need with speed you want.

Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with either a mouse or rabbit primary antibody.

Highlights:

  • Fast – femtogram levels of detection in about 1 hour
  • Convenient – no expensive hardware or vacuum required; no clogging issues
  • Simple – optimized protocol makes Western blot analysis easier than ever
  • Economical – no expensive consumables or extra equipment
  • Excellent stability – kit is stable for 1 year stored at 4°C
  • Easy – complete kits contain all components needed to block, probe and develop a blot with your mouse or rabbit primary antibody

Product Details:


Detection of GRB14 and SMAD2 with SuperSignal West Dura Fast Western Blot Kit
 
Classical vs. Fast Western protocol using Thermo Scientific SuperSignal West Dura Substrate. HeLa cell lysate was diluted in reducing sample buffer and loaded to a gel. After electrophoresis, the proteins were transferred to nitrocellulose and PVDF membranes using a semi-dry blotter for 10 minutes at 25V. Membranes were developed using the classic Western protocol or the fast Western protocol. Each blot was incubated in SuperSignal West Dura Substrate Working Solution for 5 minutes and exposed to film. GRB14 detection: The blots were probed with rabbit anti-GRB14 antibody (0.5µg/mL, Millipore) and exposed to film for 1 minute. SMAD2 detection: Blots were probed with mouse anti-SMAD2 antibody (1/1000 dilution, Cell Signaling Technology). Blots were exposed to film for 5 minutes for nitrocellulose and 1 minute for PVDF.

 

Comparision of common Western blotting protocols. Side by side comparison of the steps and times required to complete a Western blot (post transfer and before development with substrate) using a classical protocol, the reagent-based Thermo Scientific Pierce Fast Western System, and the instrument-based SNAP i.d.™ System (Millipore Corp).
Protocol Steps Classical protocol Pierce Fast System SNAP i.d.™ System
Block/Pretreat 60 min - - - -
Primary antibody 60 min 30 min 10 min
Wash 45 min - - 1 min
Secondary antibody 60 min 10 min 10 min
Wash 45 min 15 min 1 min
Total Time 270 min 55 min 22 min
Key limitations Preparation of fresh non-fat dry milk blocking buffer each time For use with mouse or rabbit primary antibodies Only 2 full-sized blots per cycle. Strips limited by the format of the blot holders available. Reliable vacuum source required

 

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate is a cost-effective means to obtain Western blot results quickly. This table provides a brief cost of reagents and required consumables used in a classical Western protocol, the Pierce Fast Western reagent-based system and Millipore SNAP i.d.™ system.
Western blot reagents Classical
Protocol
Pierce Fast
System
SNAP i.d.
System
Blot holder for instrument - - - - $7.33
Blotting membrane (NC) $2.48 $2.48 $2.48
Blocking buffer $0.02 - - $1.32
Wash buffer (TBST) $1.48 - - $1.48
Secondary antibody conjugate $0.15 - - $0.15
Detection substrate
(SuperSignal West Dura Substrate)
$26.25 - - $26.25
Film (5x7) $1.01 $1.01 $1.01
Kit's price per blot - - $33.60 - -
Total price per blot $31.43 $37.13 $40.06
Prices based on 2010 Thermo Scientific Pierce Western Blot Reagents and using SuperSignal West Dura Chemiluminescent Substrate as the detection reagent for comparable levels of sensitivity.

Related Products:

Pierce G2 Fast Blotter
Membranes and Filter Paper
CL-Xposure Film for Western blotting
Pierce Western Blot Signal Enhancer
Restore PLUS Western Blot Stripping Buffer
Browse all Pierce Western Blotting Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
35075 Fast Western Blot Kits, SuperSignal West Dura, Mouse
Formulation: Multi-component kit
Sufficient For: 2 mini blots probed with mouse primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 40mL
Fast Western 10X Wash Buffer, 20mL
Fast Western Mouse Optimized HRP Reagent, Dura, 2mL
SuperSignal West Dura Luminol/Enhancer Solution, 10mL
SuperSignal West Stable Peroxide Solution, 10mL
80mL kit Product Instructions for product #35075 Fast Western Blot Kits, SuperSignal West Dura, Mouse MSDS for product #35075 Fast Western Blot Kits, SuperSignal West Dura, Mouse Certificate of Analysis for product #35075 Fast Western Blot Kits, SuperSignal West Dura, Mouse $109.00

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35070 Fast Western Blot Kits, SuperSignal West Dura, Mouse
Formulation: Multi-component kit
Sufficient For: 10 mini blots probed with mouse primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 200mL
Fast Western 10X Wash Buffer, 100mL
Fast Western Mouse Optimized HRP Reagent, Dura, 10mL
SuperSignal West Dura Luminol/Enhancer Solution, 50mL
SuperSignal West Stable Peroxide Solution, 50mL
400mL kit Product Instructions for product #35070 Fast Western Blot Kits, SuperSignal West Dura, Mouse MSDS for product #35070 Fast Western Blot Kits, SuperSignal West Dura, Mouse Certificate of Analysis for product #35070 Fast Western Blot Kits, SuperSignal West Dura, Mouse $373.00

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35076 Fast Western Blot Kits, SuperSignal West Dura, Rabbit
Formulation: Multi-component kit
Sufficient For: 2 mini blots probed with rabbit primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 40mL
Fast Western 10X Wash Buffer, 20mL
Fast Western Rabbit Optimized HRP Reagent, Dura, 2mL
SuperSignal West Dura Luminol/Enhancer Solution, 10mL
SuperSignal West Stable Peroxide Solution, 10mL
80mL kit Product Instructions for product #35076 Fast Western Blot Kits, SuperSignal West Dura, Rabbit MSDS for product #35076 Fast Western Blot Kits, SuperSignal West Dura, Rabbit $109.00

Add to cart
35071 Fast Western Blot Kits, SuperSignal West Dura, Rabbit
Formulation: Multi-component kit
Sufficient For: 10 mini blots probed with rabbit primary antibodies

Kit Contents:
Fast Western Antibody Diluent, 200mL
Fast Western 10X Wash Buffer, 100mL
Fast Western Rabbit Optimized HRP Reagent, Dura, 10mL
SuperSignal West Dura Luminol/Enhancer Solution, 50mL
SuperSignal West Stable Peroxide Solution, 50mL
400mL kit Product Instructions for product #35071 Fast Western Blot Kits, SuperSignal West Dura, Rabbit MSDS for product #35071 Fast Western Blot Kits, SuperSignal West Dura, Rabbit Certificate of Analysis for product #35071 Fast Western Blot Kits, SuperSignal West Dura, Rabbit $373.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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