Thermo Scientific EZ-Link TFP-PEG3-Biotin is a tetrafluorophenyl-ester reagent that enables labeling at primary amines found on lysine residues and the N-terminus and secondary amines such as histidine and tryptophan.
TFP-PEG3-Biotin enables simple and efficient biotin labeling of antibodies, proteins and other amine-containing macromolecules. The tetrafluorophenyl ester (TFP ester) reacts with primary and secondary amines at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.
- Molecular labeling – biotinylate proteins and nucleic acids for avidin-biotin methods
- Amine-reactive – reacts with primary and secondary amines of proteins and other molecules (unlike typical NHS reagents, which react with primary amines only)
- Pegylated – spacer arm contains a hydrophilic, 3-unit, polyethylene glycol (PEG) group
- Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
- Irreversible – forms permanent amide bonds; spacer arm cannot be cleaved
- Long reach – spacer arm (total length added to target) is 32.6 angstroms; this reduces steric hindrance when binding to avidin molecules
|Chemical structure of TFP-PEG3-Biotin (biotin tetrafluorophenyl ester).This biotinylation reagent labels proteins, nucleic acids and other molecules that contain primary amines or secondary amines. For more information, see our review of Amine-reactive Crosslinker Chemistry.
Properties of TFP-PEG3-Biotin.
dPEG-Biotin TFP ester
|Spacer arm length
||White to tan solid
||4°C with desiccant, protect from moisture, use only fresh solutions
||Tetrafluorophenyl ester, reacts with primary amines at pH 7.0-9.0
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
- Liu, W., et al. (2004) J. Biol. Chem.279, 18748-18758.
- Kellermann, S-A. and McEvoy, L.M. (2001) J. Immunol. 167, 682-690.
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Review of Pegylation (Polyethylene Glycol)
Chemistry of Crosslinking (and Labeling Reagents)
All Amine-Reactive Biotinylation Reagents
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins