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EZ-Link Sulfo-NHS-SS-Biotin and Biotinylation Kits


For cleavable (reversible) attachment of biotin to protein and cell surface amines.

EZ-Link Sulfo-NHS-SS-Biotin

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Sulfo-NHS-SS-Biotin is a thiol-cleavable amine-reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding. This reagent is water-soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion. The compound is particularly useful for labeling and purifying cell surface proteins, because (a) its sulfonate group prevents it from permeating cell membranes and (b) its cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.

Highlights:

  • Protein labeling – biotinylate antibodies to facilitate immobilization, purification or detection
  • Cell surface labeling – biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
  • Amine-reactive – reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
  • Cleavable – disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
  • Soluble – charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
  • Medium length – spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Product Details:

Chemical structure of Sulfo-NHS-SS-Biotin
Chemical structure of Sulfo-NHS-SS-Biotin. This biotinylation reagent labels antibodies and other proteins and molecules that contain primary amines (e.g., side-chain of lysine). For more information, see our review of NHS Ester Reaction Chemistry.

Properties of Sulfo-NHS-SS-Biotin. This amine-reactive compound has a cleavable disulfide bond and is useful for cell surface protein labeling and isolation.
Molecular formula C19H27O9N4S4Na
SMILES structure O=C(ON1C(C(S([O-])(=O)=O)CC1=O)=O)CCSSCCNC(CCCCC(SC2)C3C2NC(N3)=O)=O.[Na+]
Molecular weight 606.69
Spacer arm length 24.3 angstroms
Mass added to target 389.6 daltons
Form White to off-white powder
Storage conditions 4°C with desiccant, protect from moisture, use only fresh solutions
Reactive groups N-hydroxysuccinimide ester, reacts with primary amines at pH 7.0-9.0
Cleave with 50mM DTT or other reducing agents

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.

Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.

References:

  1. Resnik N. et al. (2011) Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes. J. Biol. Chem. 286, 1499-507.
  2. Hoffmeister H. et al. (2011) Polycystin-2 takes different routes to the somatic and ciliary plasma membrane. J. Cell Biol., jcb.201007050.
  3. Suzuki M. et al. (2010) Defective membrane expression of the na+-hco3- cotransporter nbce1 is associated with familial migraine. PNAS. 107, 15963-8.
  4. Lavieu G. et al. (2010) Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes. PNAS. 107, 6876-81.
  5. Jo M. et al. (2010) Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 70, 8948-58.
  6. Howes M. T. et al. (2010) Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells. J. Cell Biol. 190, 675-91.
  7. de Miguel N. et al. (2010) Proteome analysis of the surface of trichomonas vaginalis reveals novel proteins and strain-dependent differential expression. Mol. Cell. Proteomics. 9, 1554-66.

Related Resources:

Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

Pierce Cell Surface Protein Isolation Kit
NHS-PEG4-Biotin and Kits – 29.0 angstrom spacer arm, pegylated
Sulfo-NHS-LC-Biotin and Kits – 22.4 angstrom spacer arm, non-cleavable
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21445 EZ-Link Sulfo NHS-SS Biotinylation Kit
Formulation: Multi-component kit
Sufficient For: 10 labeling reactions, each with 1 to 10mg of antibody

Kit Contents:
EZ-Link Sulfo-NHS-SS-Biotin, 25mg
PBS Pack (makes 500mL), 1 pack
Zeba Spin Desalting Columns (7K MWCO), 5mL, 10 columns
HABA Solution, 1mL
Avidin, 10mg
10-rxn kit Product Instructions for product #21445 EZ-Link Sulfo NHS-SS Biotinylation Kit MSDS for product #21445 EZ-Link Sulfo NHS-SS Biotinylation Kit Certificate of Analysis for product #21445 EZ-Link Sulfo NHS-SS Biotinylation Kit $328.00

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21945 EZ-Link Micro Sulfo-NHS-SS-Biotinylation Kit
Formulation: Multi-component kit
Sufficient For: 8 labeling reactions, each with 0.05-0.2mg of antibody

Kit Contents:
EZ-Link Sulfo-NHS-SS-Biotin, 8 x 1mg
PBS Pack (makes 500mL), 1 pack
Zeba Spin Desalting Columns (7K MWCO), 2mL, 10 columns
8-rxn kit Product Instructions for product #21945 EZ-Link Micro Sulfo-NHS-SS-Biotinylation Kit MSDS for product #21945 EZ-Link Micro Sulfo-NHS-SS-Biotinylation Kit $313.00

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21331 EZ Link Sulfo-NHS-SS-Biotin
Formulation: Sulfo-NHS ester of biotin with an ethyl-1,3-dithioproprionate spacer
Sufficient For: Approx. 50 uses to label a total of 1099mg of IgG using typical conditions
100mg Product Instructions for product #21331 EZ Link Sulfo-NHS-SS-Biotin MSDS for product #21331 EZ Link Sulfo-NHS-SS-Biotin Certificate of Analysis for product #21331 EZ Link Sulfo-NHS-SS-Biotin $239.00

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21331B EZ-Link Sulfo-NHS-SS-Biotin
Formulation: Sulfo-NHS ester of biotin with an ethyl-1,3-dithioproprionate spacer
Custom MSDS for product #21331B EZ-Link Sulfo-NHS-SS-Biotin Certificate of Analysis for product #21331B EZ-Link Sulfo-NHS-SS-Biotin
21328 EZ-Link Sulfo-NHS-SS-Biotin, No Weigh Format
Formulation: Sulfo-NHS ester of biotin with an ethyl-1,3-dithioproprionate spacer
Sufficient For: 8 uses to label a total of 88mg of IgG using typical conditions
8 x 1mg Product Instructions for product #21328 EZ-Link Sulfo-NHS-SS-Biotin, No Weigh Format MSDS for product #21328 EZ-Link Sulfo-NHS-SS-Biotin, No Weigh Format Certificate of Analysis for product #21328 EZ-Link Sulfo-NHS-SS-Biotin, No Weigh Format $159.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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