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EZ-Link Sulfo-NHS-LC-Desthiobiotin

Label soluble or cell surface proteins for purification with streptavidin beads.

EZ-Link Sulfo-NHS-LC-Desthiobiotin

Thermo Scientific EZ-Link Sulfo-NHS-LC-Desthiobiotin is a long-chain, amine-reactive labeling reagent whose biotin-like group is elutable from streptavidin, making it ideal for purified or cell surface protein labeling and purification.

Sulfo-NHS-LC-Desthiobiotin is a variant of biotin that is activated as a sulfo-NHS ester with a long-chain (LC) spacer arm to covalently label primary amines (-NH2) of proteins or other molecules with desthiobiotin groups. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Sulfo-NHS-LC-Biotin (Part No. 21335) for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. The negatively charged sulfo-NHS group prevents this reagent from permeating cell membranes; therefore, Sulfo-NHS-LC-Desthiobiotin can be used to confine labeling to cell surface proteins of intact cells. The No-Weigh Format (5 x 1mg resealable vials) eliminates the difficulties associated with weighing small quantities of reagent and maximizes protection of unused reagent from hydrolysis.


  • Desthiobiotin – analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
  • Protein labeling – tag antibodies or proteins in purified or mixed samples to enable high recovery in pull-down assays with streptavidin beads
  • Cell surface labeling – modifies only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
  • Amine-reactive – reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
  • Soluble – charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds

Product Details:

Chemical structure of NHS-SS-Biotin
Chemical structure of Sulfo-NHS-LC-Desthiobiotin. This reagent labels antibodies and other proteins and molecules that contain primary amines (e.g., side-chain of lysine). For more information, see our review of NHS Ester Reaction Chemistry.

Properties of Sulfo-NHS-LC-Desthiobiotin. We manufacture our reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Molecular formula C20H31N4NaO9S
SMILES structure CC(C(CCCCCC(NCCCCCC(ON1C(CC(S(=O)([O-])=O)C1=O)=O)=O)=O)N2)NC2=O.[Na+]
Molecular weight 526.54
Spacer arm length 17.3 angstroms
Mass added to target 310.20 daltons
Storage conditions -20°C with desiccant, protect from moisture, use only fresh solutions
Reactive groups N-hydroxysuccinimide ester, reacts with primary amines at pH 7.0-9.0

Desthiobiotin vs. Biotin:

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (1/Kd = 10^11 vs. 10^15M, respectively)[1-2]. Consequently, desthiobiotinylated bait proteins and their interacting partners can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down assay experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. The modified avidin-biotin affinity system also eliminates the need to use harsh elution conditions that might disassociate complexes and/or damage the target protein or cell. Desthiobiotin-based techniques are ideal when using native or recombinant proteins that are not expressed with a fusion tag and when isolating captured proteins under native conditions, such as targeting intact cells or cell surface proteins.

Labeling with NHS Ester Reagents:

N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide. Most sulfo-NHS esters are directly water soluble but not membrane permeable. Plain NHS esters are less soluble in aqueous buffers but are membrane permeable.

Cited References:

  1. Hirsch, J., et al. (2002). Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation. Analytical Biochemistry 308: 343-357.
  2. Hofmann, K., et al. (1982). Avidin binding of carboxyl-substituted biotin and analogues. Biochemistry 21: 978-984.

Related Resources:

Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

Desthiobiotin Product Selection Guide
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
16136 EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format
Formulation: Sulfo-NHS ester of desthiobiotin with a 6-aminocaproic acid spacer
Sufficient For: 5 uses to label a total of 63mg of IgG using typical conditions
5 x 1mg Product Instructions for product #16136 EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format MSDS for product #16136 EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format Certificate of Analysis for product #16136 EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format $282.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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