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EZ-Link Psoralen-PEG3-Biotin


Light-activates to intercalate and label RNA or DNA.

EZ-Link Psoralen-PEG3-Biotin

Thermo Scientific EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form a covalent bonds.

Psoralen-PEG3-Biotin is a photoactivatable reagent for biotinylation of DNA or RNA. Upon activation with UV-Light (~350nm), the psoralen group reacts with thymine- and other pyrimidine-containing bases to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Highlights:

  • Biomolecular labeling – biotinylate DNA or RNA (approximately 1 biotin per 10 to 20 bases) without interfering with hybridization
  • Photo-reactive – covalent attachment occurs in the presence of UV light (~350nm) for 10 to 30 minutes
  • Psoralen-activated – reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
  • Pegylated – spacer arm contains a hydrophilic, 3-unit, polyethylene glycol (PEG) group
  • Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
  • Long reach – spacer arm (total length added to target) is 36.9 angstroms, minimizing steric hindrance for binding interactions with streptavidin

Product Details:

Chemical structure of Psoralen-PEG3-Biotin
Chemical structure of Psoralen-PEG3-Biotin. This biotinylation reagent covalently attaches to nucleic acids upon photo-activation with UV-light. For more information, see our review of Photoreactive Conjugation Chemistry.

Properties of Psoralen-PEG3-Biotin. This photo-activatable compound has an extended, pegylated spacer arm terminated by a psoralen reactive group.
SMILES structure:
O=C1OC2=C(OCCCC(NCCOCCOCCOCCNC(CCCCC(SC3)C4C3NC(N4)=O)=O)=O)C5=C(C=CO5)C=C2C=C1
Molecular formula C33H44N4O10S
Molecular weight 688.79
Spacer arm length 36.9 angstroms
Form White to off-white solid
Storage conditions 4°C protected from light
Reactive groups Psoralen, reacts upon exposure to ultraviolet light

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Applications:

  • Selective labeling of cell surface proteins (Ref.1)
  • Biotinylation of hyaluronan (Ref.2)
  • Labeling of endotoxin for receptor binding studies (Ref.3)

References:

  • Henriksen, U., et al. (1991). J. Photochem. Photobiol. A: Chem. 57, 331-342.
  • Oser, A., et al. (1988). Nucleic Acids Res. 16, 1181-1196.
  • Wassarman, D.A. (1993). Mol. Biol. Rep. 17, 143-151.
  • Cimono, G.D., et al. (1985). Annu. Rev. Biochem. 54, 1151-1193.
  • Wassarman, D.A. (1993). Mol. Biol. Rep. 17, 143-151.

Related Resources:

Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
29986 EZ-Link Psoralen-PEG3-Biotin
Formulation: Psoralen-activated biotin with a triethyleneglycol spacer
Sufficient For: 1 or 2 uses to prepare a total of 36mL of typical 1X (0.2mM) reaction solution with 20-100µg/mL DNA
5mg Product Instructions for product #29986 EZ-Link Psoralen-PEG3-Biotin MSDS for product #29986 EZ-Link Psoralen-PEG3-Biotin Certificate of Analysis for product #29986 EZ-Link Psoralen-PEG3-Biotin $243.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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