Thermo Scientific EZ-Link Photoactivatable Biotin is a nitrophenyl azide biotin compound that forms covalent bonds with molecules upon activation by exposure to ultraviolet light.
We no longer recommend this product because a newer, more effective photoreactive biotinylation reagent is available. TFPA-PEG3-Biotin (Part No. 21303) has a more efficient reactive group and a more soluble and a flexible spacer arm.
- Covalent attachment occurs in the presence of UV light (350-370nm)
- Attachment is nonspecific; can even replace C-H bonds or C-C bonds
- Should be dissolved in water or 0.1 M EDTA, pH 8 before use
- Reaction buffer should be above pH 6.0
|Chemical structure of Photoactivatable Biotin. This biotinylation reagent covalently attaches to proteins and other molecules upon photo-activation with UV-light. For more information, see our review of Aryl Azide Reaction Chemistry.
- Stable nucleic acid labeling for in situ, Southern, Northern and dot blot hybridization techniques
- Tubulin has been biotinylated without loss of binding activity
- Forster, A.C., et al. (1985). Nucleic Acids Res. 13, 745-761.
- Lacey, E. and Grant, W.N. (1987). Anal. Biochem. 163, 151-158.
- Smith, J.S., et al. (1999). Proc. Natl. Acad. Sci. USA 96(16), 8855-8860.
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins