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EZ-Link Photoactivatable Biotin


Eliminates the need for radioactive, short half-lived DNA probes.

Thermo Scientific EZ-Link Photoactivatable Biotin is a nitrophenyl azide biotin compound that forms covalent bonds with molecules upon activation by exposure to ultraviolet light.

We no longer recommend this product because a newer, more effective photoreactive biotinylation reagent is available. TFPA-PEG3-Biotin (Part No. 21303) has a more efficient reactive group and a more soluble and a flexible spacer arm.

Highlights:

  • Covalent attachment occurs in the presence of UV light (350-370nm)
  • Attachment is nonspecific; can even replace C-H bonds or C-C bonds
  • Should be dissolved in water or 0.1 M EDTA, pH 8 before use
  • Reaction buffer should be above pH 6.0

Product Details:

Chemical structure of Photoactivatable-Biotin
Chemical structure of Photoactivatable Biotin. This biotinylation reagent covalently attaches to proteins and other molecules upon photo-activation with UV-light. For more information, see our review of Aryl Azide Reaction Chemistry.

Applications:

  • Stable nucleic acid labeling for in situ, Southern, Northern and dot blot hybridization techniques
  • Tubulin has been biotinylated without loss of binding activity

References:

  1. Forster, A.C., et al. (1985). Nucleic Acids Res. 13, 745-761.
  2. Lacey, E. and Grant, W.N. (1987). Anal. Biochem. 163, 151-158.
  3. Smith, J.S., et al. (1999). Proc. Natl. Acad. Sci. USA 96(16), 8855-8860.

Related Resources:

Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

TFPA-PEG3-Biotin (recommended)
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
29987 EZ-Link Photoactivatable Biotin 0.5mg MSDS for product #29987 EZ-Link Photoactivatable Biotin Certificate of Analysis for product #29987 EZ-Link Photoactivatable Biotin $144.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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