Update profile
    Change password
    Sign out

EZ-Link Phosphine-PEG4-Desthiobiotin


Label azide-tagged groups for affinity purification with streptavidin beads.

EZ-Link Phosphine-PEG4-Desthiobiotin

Thermo Scientific EZ-Link Phosphine-PEG4-Desthiobiotin is a pegylated reagent whose biotin-like group is elutable from streptavidin, activated for chemoselective ligation and purification of azide targets tagged by metabolic labeling.

When used in combination with azide labeling strategies, Phosphine-PEG4-Desthiobiotin enables affinity purification of protein interactions and post-translational modifications using streptavidin resins. The phosphine moiety selectively crosslinks via the Staudinger reaction mechanism to molecules that have been tagged or metabolically labeled with bioorthogonal azide groups. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Phosphine-PEG3-Biotin (Part No. 88901) for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. The No-Weigh Format (5 x 1mg resealable vials) eliminates the difficulties associated with weighing small quantities of reagent and maximizes protection of unused reagent from degradation.

Highlights:

  • Desthiobiotin – analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
  • Chemoselective – the phosphine reactive group is specific in biological samples for azide-tagged molecules, ensuring specific capture and purification of labeled targets
  • Compatible – Staudinger reaction chemistry proceeds efficiently in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
  • PEG spacer – polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein
  • Soluble – easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples

Product Details:

Chemical structure of NHS-SS-Biotin
Chemical structure of Phosphine-PEG4-Desthiobiotin. This reagent is for labeling and detection of azide-tagged molecules via the Staudinger ligation reaction chemistry.

 
Properties of Phosphine-PEG4-Desthiobiotin. We manufacture our reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Molecular formula C41H55N4O9P
SMILES structure CC(C(CCCCCC(NCCOCCOCCOCCOCCNC(C1=CC(P(C2=CC=CC=C2)C3=CC=CC=C3) =C(C(OC)=O)C=C1)=O)=O)N4)NC4=O
Molecular weight 778.87
Spacer arm length 30.6 angstroms
Mass added to target 747.87 daltons
Storage conditions 4°C

Desthiobiotin vs. Biotin:

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (1/Kd = 10^11 vs. 10^15M, respectively)[1-2]. Consequently, desthiobiotinylated bait proteins and their interacting partners can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down assay experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. The modified avidin-biotin affinity system also eliminates the need to use harsh elution conditions that might disassociate complexes and/or damage the target protein or cell. Desthiobiotin-based techniques are ideal when using native or recombinant proteins that are not expressed with a fusion tag and when isolating captured proteins under native conditions, such as targeting intact cells or cell surface proteins.

Labeling with Phosphine Reagents:

Azide groups can be introduced into proteins or other cellular targets through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks. Because phosphines and azides are foreign and invisible to biological systems (bioorthogonal), they comprise a chemoselective (mutually specific) ligation pair for labeling and conjugation.

Overview of Staudinger ligation for biomolecular conjugation.
Overview of Staudinger ligation (azide-phosphine conjugation). Phosphine-activated compounds conjugate with high specificity to azide-tagged molecules, resulting in stable covalent attachment of "A" and "B" molecules. For additional information, see our review of Staudinger ligation reaction chemistry.

Cited References:

  1. Hirsch, J., et al. (2002). Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation. Analytical Biochemistry 308: 343-357.
  2. Hofmann, K., et al. (1982). Avidin binding of carboxyl-substituted biotin and analogues. Biochemistry 21: 978-984.

Related Resources:

Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

Azide-Phosphine Reagent Selection Guide
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
16133 EZ-Link Phosphine-PEG4-Desthiobiotin, No-Weigh Format
Formulation: Phosphine-activated desthiobiotin with a tetraethyleneglycol spacer
Sufficient For: 5 uses to label a total of 32mg of IgG using typical conditions
5 x 1mg Product Instructions for product #16133 EZ-Link Phosphine-PEG4-Desthiobiotin, No-Weigh Format MSDS for product #16133 EZ-Link Phosphine-PEG4-Desthiobiotin, No-Weigh Format Certificate of Analysis for product #16133 EZ-Link Phosphine-PEG4-Desthiobiotin, No-Weigh Format $545.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747